Influence of the application of chelant EDDS on soil enzymatic activity and microbial community structure.
Influence of the application of chelant EDDS on soil enzymatic activity and microbial community structure.
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DOI：
10.1016/j.jhazmat.2013.09.009
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发表时间：
2013-11
影响因子：
13.6
通讯作者：
L. Yang;Gui-ping Wang;Zhineng Cheng;Yue Liu;Z. Shen;Chunling Luo
中科院分区：
文献类型：
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作者：
L. Yang;Gui-ping Wang;Zhineng Cheng;Yue Liu;Z. Shen;Chunling Luo
本研究评价了生物可降解螯合剂S，S-乙二胺二琥珀酸（EDDS）对铜（Cu）污染土壤中酶活性和微生物群落组成的影响。结果表明，施用EDDS对土壤脲酶和酸性磷酸酶活性没有影响，但在施用EDDS后第7天，土壤过氧化氢酶和蔗糖酶活性显著降低，β-葡萄糖苷酶活性升高。在第28天，EDDS处理的土壤中的酶活性（除β-葡萄糖苷酶外）与对照相比没有显著差异。磷脂脂肪酸（PLFAs）分析表明，施用3 mmol kg− 1 EDDS不会对土壤微生物群落造成明显的胁迫。PCR-DGGE指纹图谱显示，EDDS对土壤细菌群落结构有影响，且对大豆土壤的影响大于玉米土壤。总的来说，EDDS的施用对土壤酶活性和细菌群落有一定的影响，但随着EDDS的降解，这种影响逐渐减弱甚至消失。
The present study evaluated the effects of a biodegradable chelant, S,S-ethylenediaminedisuccinic acid (EDDS), on enzyme activities and microbial community composition in copper (Cu)-contaminated soils, planted with either corn or beans. Results showed that the application of EDDS did not affect urease and acid phosphatase activities in the soil, but greatly reduced catalase and saccharase activities, and increased β-glucosidase activity on the seventh day after EDDS application. On the 28th day, no significant difference was observed in the enzyme activities (except for β-glucosidase) of EDDS-treated soils compared to the controls. Analysis of phospholipid fatty acids (PLFAs) showed that the application of 3 mmol kg−1EDDS did not cause significant stress to soil microbial communities. However, PCR-denaturing gradient gel electrophoresis (PCR-DGGE) fingerprint revealed that EDDS influenced the bacterial communities in the soils, and the effects on bean soils were more significant than that with corn. In general, the enzyme activities and bacterial communities were influenced by the application of EDDS, but the impact became weaker or even disappeared with the biodegradation of EDDS.