The use of electron tomography for structural analysis of disordered protein arrays
The use of electron tomography for structural analysis of disordered protein arrays
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DOI：
10.1006/jsbi.1997.3932
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发表时间：
1997-12-01
影响因子：
3
通讯作者：
Winkler, H
中科院分区：
文献类型：
--
作者：
Taylor, KA;Tang, JH;Winkler, H
本文提出了一种基于傅里叶变换的方法，用于从具有静态无序的次晶物体中获得三维重建。该方法是从标准方法中使用的2-D晶体的3-D重建，除了所有的傅立叶系数被使用，而不仅仅是从周期性晶格采样数据，因此，不仅是在3-D可视化的结构的空间有序的部分，而且空间无序的部分。应用该方法的昆虫飞行肌肉的3-D重建的描述，以及该方法的扩展到辐射敏感的标本的前景。(C)北京：科学出版社.
A method based on Fourier transforms is described for obtaining a 3-D reconstruction from a paracrystalline object with static disorder. The method is derived from the standard methods used in 3-D reconstruction of 2-D crystals except that all of the Fourier coefficients are used and not just the sampled data from the periodic lattice, Thus, not only is the spatially ordered part of the structure visualized in 3-D, but also the spatially disordered part. Application of the method to 3-D reconstructions of insect flight muscle is described as well as prospects for extension of the method to radiation-sensitive specimens. (C) 1997 Academic Press.