Assignment of microsomal epoxide hydrolase (EPHX1) to human chromosome 1q42.1 by in situ hybridization
Assignment of microsomal epoxide hydrolase (EPHX1) to human chromosome 1q42.1 by in situ hybridization
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DOI：
10.1159/000015164
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Saari, JA
中科院分区：
文献类型：
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作者：
Hartsfield, JK;Sutcliffe, MJ;Saari, JA
材料和方法从植物血凝素刺激的淋巴细胞中制备具有正常核型的人中期细胞，并如Buckle和Rack（1993）所述进行FISH。G11的插件。1进行凝胶纯化并用生物素-14-dATP（Life Technologies/Gibco BRL，Grand Island NY）标记。杂交探针用荧光素标记的亲和素检测。
Materials and methodsHuman metaphase cells with a normal karyotype were prepared from phytohemagglutinin-stimulated lymphocytes and FISH was performed as described by Buckle and Rack (1993). The insert from G11. 1 was gel purified and labeled with biotin-14-dATP (Life Technologies/Gibco BRL, Grand Island NY). The hybridized probe was detected with fluorescein conjugated avidin.