PHYLOGENY AND EXPRESSION OF AXONEMAL AND CYTOPLASMIC DYNEIN GENES IN SEA-URCHINS
PHYLOGENY AND EXPRESSION OF AXONEMAL AND CYTOPLASMIC DYNEIN GENES IN SEA-URCHINS
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DOI：
10.1091/mbc.5.1.57
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发表时间：
1994-01-01
影响因子：
3.3
通讯作者：
GIBBONS, IR
中科院分区：
文献类型：
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作者：
GIBBONS, BH;ASAI, DJ;GIBBONS, IR
海胆胚胎的 Poly(A)+ RNA 中已鉴定出编码动力蛋白重链的 14 个不同基因的长度类似于 14.5 kb 的转录本。对这些动力蛋白转录物水平随脱纤毛的变化及其序列相关性的分析表明，这些基因中的 11 个或更多编码参与胚胎外部纤毛再生的动力蛋白亚型，而推导的序列与其他生物体中细胞质动力蛋白的序列非常相似的单个基因似乎不参与这种再生。先前在海胆动力蛋白β重链中发现的磷酸盐结合的四个共有基序存在于已获得延伸序列的所有其他五种亚型中，表明这些位点在动力蛋白功能中发挥着重要作用。对包含假定的水解 ATP 结合位点的类似 400 个氨基酸区域的序列分析表明，动力蛋白基因至少分为六个不同的类别。海胆中的大多数这些类别与果蝇中鉴定的动力蛋白重链基因之一具有高度的序列同一性，表明动力蛋白基因家族向现有类别的辐射发生在真核生物进化的早期阶段。细胞质动力蛋白的进化变化比轴丝动力蛋白的进化变化受到更多限制。
Transcripts similar to 14.5 kilobases in length from 14 different genes that encode for dynein heavy chains have been identified in poly(A)+ RNA from sea urchin embryos. Analysis of the changes in level of these dynein transcripts in response to deciliation, together with their sequence relatedness, suggests that 11 or more of these genes encode dynein isoforms that participate in regeneration of external cilia on the embryo, whereas the single gene whose deduced sequence closely resembles that of cytoplasmic dynein in other organisms appears not to be involved in this regeneration. The four consensus motifs for phosphate binding found previously in the beta heavy chain of sea urchin dynein are present in all five additional isoforms for which extended sequences have been obtained, suggesting that these sites play a significant role in dynein function. Sequence analysis of a similar to 400 amino acid region encompassing the putative hydrolytic ATP-binding site shows that the dynein genes fall into at least six distinct classes. Most of these classes in sea urchin have a high degree of sequence identity with one of the dynein heavy chain genes identified in Drosophila, indicating that the radiation of the dynein gene family into the present classes occurred at an early stage in the evolution of eukaryotes. Evolutionary changes in cytoplasmic dynein have been more constrained than those in the axonemal dyneins.