Electron microscopic evidence of persistent chlamydial infection following treatment
Electron microscopic evidence of persistent chlamydial infection following treatment
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DOI：
10.1046/j.1468-3083.2001.00342.x
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发表时间：
2001-09-01
影响因子：
9.2
通讯作者：
Dmitriev, GA
中科院分区：
文献类型：
--
作者：
Bragina, EY;Gomberg, MA;Dmitriev, GA
女性和男性生殖道的沙眼衣原体感染通常无症状，因此往往会持续存在。在持续状态下，典型的衣原体生命周期被阻止，标准的抗生素方案并不总是能根除这种感染。我们试图将男性和女性生殖道持续性衣原体感染的治疗失败与衣原体持续存在的电子显微镜证据和生物体的非典型形态学形式联系起来。在阿奇霉素治疗后衣原体持续存在的16例患者中，通过电子显微镜从一个宫颈内样本和一个男性尿道样本中观察到这种生物体的形态学变体。我们的文件中存在的细胞内包涵体只含有网状机构，细胞外的单膜和多膜吞噬体含有初级机构和网状机构与异常外膜在细胞外分裂的过程中。这些观察结果与先前在不利条件下衣原体持续存在的体外研究结果一致。这种能力的C。沙眼衣原体在体内经历非典型形态学改变可能有助于其持久性和对抗生素的相对耐药性。
Chlamydia trachomatis infections of the female and male genital tracts are often asymptomatic and, thus, tend to become persistent. In the persistent state the typical Chlamydia life cycle is arrested and standard antibiotic regimens do not always eradicate this infection. We sought to relate treatment failures in men and women with persistent chlamydial genital tract infections to electron microscopic evidence of chlamydial persistence and with atypical morphological forms of the organism. Of 16 patients with chlamydial persistence following azithromycin treatment, morphological variants of this organism were observed by electron microscopy from one endocervical sample and one male urethral sample. We document the presence of intracellular inclusions containing only reticulate bodies, extracellular monomembrane and polymembrane phagosomes containing elementary bodies and reticulate bodies with abnormal outer membranes in the process of dividing extracellularly. These observations parallel previous in vitro studies of chlamydial persistence under adverse conditions. This capacity of C. trachomatis to undergo atypical morphological alterations in vivo may contribute to its persistence and relative resistance to antibiotics.