ANTIOXIDANT, GALLIC ACID, INDUCES APOPTOSIS IN HL-60RG CELLS
ANTIOXIDANT, GALLIC ACID, INDUCES APOPTOSIS IN HL-60RG CELLS
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DOI：
10.1006/bbrc.1994.2544
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发表时间：
1994-10-28
影响因子：
3.1
通讯作者：
YABU, Y
中科院分区：
文献类型：
--
作者：
INOUE, M;SUZUKI, R;YABU, Y
没食子酸是一种天然存在的具有抗氧化活性的植物酚，已被发现能诱导早幼粒白血病HL-60RG细胞死亡，虽然许多抗氧化剂都能保护细胞免受氧化应激的伤害，但形态和生化研究表明，没食子酸诱导的细胞死亡是细胞凋亡。流式细胞仪分析显示，HL-60RG细胞在细胞周期的第一个特定时相不会触发细胞凋亡，而没食子酸作用HL-60RG细胞2 h就足以诱导细胞凋亡。抑制实验表明，没食子酸对细胞有诱导作用。死亡由过氧化氢、超氧阴离子、钙离子、钙调素依赖的酶等活性氧物种介导。结构-活性分析表明，没食子酸诱导HL-60RG细胞凋亡，这取决于其独特的结构特征，而不是其抗氧化活性。(C)1994年学术出版社。
Gallic acid, a naturally occurring plant phenol with antioxidative activity, was found to induce cell death in promyelocytic leukemia HL-60RG cells, although many antioxidants are well known to protect the cell from oxidative stress, Morphological and biochemical studies indicated that the gallic acid-induced cell death is apoptosis. Flow cytometric analysis revealed that the apoptosis was not triggered at st specific phase of the cell cycle and that 2 h exposure of gallic acid to HL-60RG cells was enough to induce apoptosis. The inhibitory assay suggested that gallic acid-induced cell. death was mediated by reactive oxygen species such as hydrogen peroxide, superoxide anion in addition to Ca2+ ion, calmodulin-dependent enzymes. Structure-activity analysis suggests that gallic acid induces apoptosis in HL-60RG cells, depending on its distinctive feature derived from the structure but not on its antioxidative activity. (C) 1994 Academic Press, Inc.