The auxin-inducible phosphate transporter AsPT5 mediates phosphate transport and is indispensable forarbuscule formation in Chinese milk vetch atmoderately high phosphate supply
The auxin-inducible phosphate transporter AsPT5 mediates phosphate transport and is indispensable forarbuscule formation in Chinese milk vetch atmoderately high phosphate supply
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DOI：
10.1111/1462-2920.14952
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Xianan Xie
中科院分区：
文献类型：
--
作者：
Xiaoning Fan;Xianrong Che;Wenzhen Lai;Sijia Wang;Wentao Hu;Hui Chen;Bin Zhao;Ming Tang;Xianan Xie
Phosphorus is a macronutrient that is essential forplant survival. Most land plants have evolved the abil-ity to form a mutualistic symbiosis with arbuscularmycorrhizal (AM) fungi, which enhances phosphate (Pi) acquisition. Modulation of Pi transporter systemsis the master strategy used by mycorrhizal plants to adapt to ambient Pi concentrations. However, the specific functions of PHOSPHATE TRANSPORTER 1(PHT1) genes, which are Pi transporters that are responsive to high Pi availability, are largely unknown. Here, we report that AsPT5, an Astragalus sinicus (Chinese milk vetch) member of the PHT1 gene family, is conserved across dicotyledons and is constitutively expressed in a broad range of tissues independently of Pi supply, but is remarkably induced by indole-3-acetic acid (auxin) treatment under moderately high Pi conditions. Subcellular localization experiments indicated that AsPT5 localizes to the plasma membrane of plant cells. Using reverse genetics, we showed that AsPT5 not only mediates Pi transport and remodels root system architecture but is also essential for arbuscule formation in A. sinicus under moderately high Pi concentrations. Overall, our study provides insight into the function of AsPT5 in Pi transport, AM development and the cross-talk between Pi nutrition and auxin signalling in mycorrhizal plants.
