LAMININ ENHANCES BINDING OF TOXOPLASMA-GONDII TACHYZOITES TO J774 MURINE MACROPHAGE CELLS
LAMININ ENHANCES BINDING OF TOXOPLASMA-GONDII TACHYZOITES TO J774 MURINE MACROPHAGE CELLS
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DOI：
10.1128/iai.60.6.2337-2342.1992
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发表时间：
1992-06-01
影响因子：
3.1
通讯作者：
JOINER, KA
中科院分区：
文献类型：
--
作者：
FURTADO, GC;SLOWIK, M;JOINER, KA
我们研究了细胞外基质蛋白层粘连蛋白和纤连蛋白对刚地弓形虫速殖子与小鼠巨噬细胞系J774黏附的影响。层粘连蛋白而非纤连蛋白以剂量依赖的方式增加了寄生虫对J774细胞的黏附。环状YIGSR是一种源自层粘连蛋白的肽，它能抑制层粘连蛋白与宿主细胞上32/67 - kDa层粘连蛋白结合蛋白的结合，该肽阻断了层粘连蛋白介导的寄生虫黏附增强作用。一种针对32/67 - kDa层粘连蛋白结合蛋白的抗血清也抑制了寄生虫与J774细胞的结合。这些结果，结合我们之前的观察（G. C. 富尔塔多、F. L. 柯林斯和K. A. 乔伊纳，已投稿待发表），表明带有表面层粘连蛋白的速殖子在黏附不同靶细胞时与多种层粘连蛋白受体结合。
We investigated the effects of the extracellular matrix proteins laminin and fibronectin on the attachment of tachyzoites of Toxoplasma gondii to the murine macrophage cell line J774. Laminin but not fibronectin increased parasite attachment to J774 cells in a dose-dependent fashion. Cyclic YIGSR, a laminin-derived peptide which inhibits laminin binding to the 32/67-kDa laminin-binding protein on host cells, blocked the laminin-mediated enhancement of parasite attachment. An antiserum to the 32/67-kDa laminin-binding protein also inhibited binding of parasites to J774 cells. These results, in conjunction with our previous observations (G. C. Furtado, F. L. Collins, and K. A. Joiner, submitted for publication), demonstrate that tachyzoites bearing surface laminin bind to multiple laminin receptors in attaching to different target cells.