Preparation and characterization of chitosan/poly(vinyl alcohol) chemically crosslinked blends for biomedical applications
Preparation and characterization of chitosan/poly(vinyl alcohol) chemically crosslinked blends for biomedical applications
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DOI：
10.1016/j.carbpol.2008.11.015
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发表时间：
2009-04-09
影响因子：
11.2
通讯作者：
Mansur, Herman S.
中科院分区：
文献类型：
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作者：
Costa-Junior, Ezequiel S.;Barbosa-Stancioli, Edel F.;Mansur, Herman S.
在这项研究中，报道了基于壳聚糖和聚乙烯醇并通过戊二醛化学交联的新型聚合物共混物的开发和表征，该聚合物共混物可能用于各种生物医学应用。通过应力应变拉伸试验评估杂化材料的机械性能。此外，还通过 X 射线衍射、傅里叶变换红外光谱和扫描电子显微镜分析对混合水凝胶的微观结构、形貌和结晶度进行了表征。此外，还通过模拟体液溶胀试验、细胞培养MTT测定来进行生物相容性、细胞毒性和细胞活力。当聚合物网络被戊二醛网状化时，纯壳聚糖膜的相对结晶度从大约23%降低到18%。 PVA 薄膜也观察到类似的趋势。研究发现，通过增加相对于 PVA 的壳聚糖含量，共混物的溶胀指数从约 200%（纯 PVA）降低至约 80%（50/50 壳聚糖/PVA），反映了聚合物网络流动性和共混物亲水行为的降低。通过改变共混物组成和化学交联，聚合物的机械性能也得到显着改变。壳聚糖薄膜的最大拉伸强度和拉伸伸长率分别为 50 MPa 和 45%。经证实，由于壳聚糖的脆性特性，当壳聚糖浓度从25%（14.8 MJ/m(3)）增加到50%（8.9 MJ/m(3)）时，平均共混物韧性降低了约40%。另外，获得的弹性模量结果为：PVA为0.1GPa，壳聚糖为0.8GPa。测试的水凝胶清楚地显示出足够的细胞活力、无毒性和合适的机械性能，可根据皮肤工程应用的潜在用途进行定制。 (C) 2008 Elsevier Ltd. 保留所有权利。
in this study the development and characterization of novel polymer blends based on chitosan and poly (vinyl alcohol) and chemically crosslinked by glutaraldehyde for possible use in a variety of biomedical applications is reported. Mechanical properties of hybrids were evaluated by stress-strain tensile tests. Also, the microstructure, morphology and crystallinity of the blended hydrogels were characterized through X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy analysis. Moreover, biocompatibility, cytotoxicity and cell viability were also performed by swelling test in simulated body fluid, MTT assay with cell culture. The relative crystallinity of pure chitosan film was reduced from approximately 23% to 18% when the polymeric network was reticulated by glutaraldehyde. A similar trend was also observed for PVA films. It was found that by increasing the chitosan content relative to PVA the swelling index of the blend has decreased from about 200% (pure PVA) to approximately 80% (50/50 chitosan/PVA), reflecting the reduction in the mobility of the polymer network and the hydrophilic behavior of the blend. The mechanical properties of the polymers were also significantly altered by changing blend composition and chemical crosslinking. Chitosan films have shown values of 50 MPa and 45% for maximum tensile strength and tensile elongation, respectively. It was verified that the average blend toughness has decreased about 40% by increasing the chitosan concentration from 25% (14.8 MJ/m(3)) to 50% (8.9 MJ/m(3)) related to a brittle characteristic of chitosan. In addition, the obtained results of elastic moduli were 0.1 GPa for PVA and 0.8 GPa for chitosan. The tested hydrogels clearly show adequate cell viability, non-toxicity and suitable mechanical properties which can be tailored to for potential use in skin engineering applications. (C) 2008 Elsevier Ltd. All rights reserved.