EGF and insulin action in fibroblasts
EGF and insulin action in fibroblasts
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EGF 和胰岛素在成纤维细胞中的作用
DOI：
10.1016/0014-5793(86)80354-4
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发表时间：
1986
期刊：
影响因子：
3.5
通讯作者：
J. Pouysségur
中科院分区：
文献类型：
--
作者：
G. l'Allemain;J. Pouysségur
中国仓鼠肺成纤维细胞(CHL)在G0中被血清饥饿停滞，在EGF、凝血酶或血清的作用下重新启动DNA合成。当用凝血酶或血清刺激时，被[~3H]肌醇预先标记到平衡的停滞细胞，并接受20 mM LiCl预先刺激，迅速释放大量肌醇磷酸。与之形成鲜明对比的是，无论是在EGF刺激的早期或晚期，还是在添加EGF的无血清培养液中，EGF单独或联合胰岛素都不能诱导生长细胞中肌醇磷脂的分解。然而，在EGF刺激的所有阶段，磷脂酶C仍可被凝血酶高度激活。由于EGF和凝血酶是CHL的同等有效的有丝分裂原，我们得出结论，多磷肌醇的水解并不是致力于DNA复制和细胞分裂的唯一信号通路。
Chinese hamster lung fibroblasts (CHL) arrested in G0by serum starvation reinitiate DNA synthesis in response to either EGF, thrombin or serum. Arrested cells, prelabelled to equilibrium with [3H]inositol and receiving 20 mM LiCl prior stimulation, released rapidly large amounts of inositol phosphates when stimulated with thrombin or serum. In sharp contrast, EGF alone, or in association with insulin, failed to induce phosphoinositide breakdown at either early or late stages of EGF stimulation or in growing cells in EGF‐supplemented serum‐free medium. Phospholipase C remained, however, highly activatable by thrombin at all stages of EGF stimulation. Since EGF and thrombin are equally potent mitogens for CHL, we conclude that hydrolysis of polyphosphoinositides is not an exclusive signalling pathway for commitment to DNA replication and cell division.
DOI：
10.1016/s0021-9258(19)70391-3
发表时间：
1980-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Loretta M. HallcherS;W. Sherman
通讯作者：
Loretta M. HallcherS;W. Sherman