Overview of manual and automated DNA sequencing by the dideoxy chain termination method

Overview of manual and automated DNA sequencing by the dideoxy chain termination method
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双脱氧链终止法手动和自动 DNA 测序概述

DOI:
10.1016/s1046-2023(05)80158-6
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发表时间:
1991
期刊:
影响因子:
4.8
通讯作者:
Angela L. Lee
Angela L. Lee
中科院分区:
生物学3区
文献类型:
--
作者:
E. Chen;W. Kuang;Angela L. Lee

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自双脱氧链终止法首次引入以来,DNA测序经历了快速的改进,主要是在DNA聚合酶、脱氧核苷酸类似物和聚丙烯酰胺凝胶电泳领域。在这篇文章中,我们回顾了这些改进和它们的应用手动DNA测序,我们提出了一个简单的商业努力,自动化DNA样品的制备,分离和数据采集的评估。包括目前可用的仪器以及可能适合自动化的新概念。
Since the initial introduction of the dideoxy chain termination method, DNA sequencing has undergone rapid improvement, mainly in the areas of DNA polymerases, deoxynucleotide analogs, and polyacrylamide gel electrophoresis. In this article we review these improvements and their applications to manual DNA sequencing, and we present a brief evaluation of commercial efforts to automate DNA sample preparation, separation, and data acquisition. Included are instruments currently available as well as new concepts potentially amenable to automation.
DOI: --
发表时间: 1990
期刊: The Journal of biological chemistry
影响因子: --
作者:
Tabor,S;Richardson,CC
通讯作者: Richardson,CC