Overview of manual and automated DNA sequencing by the dideoxy chain termination method
Overview of manual and automated DNA sequencing by the dideoxy chain termination method
复制标题
双脱氧链终止法手动和自动 DNA 测序概述
DOI:
10.1016/s1046-2023(05)80158-6
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发表时间:
1991
期刊:
影响因子:
4.8
通讯作者:
Angela L. Lee
中科院分区:
文献类型:
--
作者:
E. Chen;W. Kuang;Angela L. Lee
Since the initial introduction of the dideoxy chain termination method, DNA sequencing has undergone rapid improvement, mainly in the areas of DNA polymerases, deoxynucleotide analogs, and polyacrylamide gel electrophoresis. In this article we review these improvements and their applications to manual DNA sequencing, and we present a brief evaluation of commercial efforts to automate DNA sample preparation, separation, and data acquisition. Included are instruments currently available as well as new concepts potentially amenable to automation.
DOI:
--
发表时间:
1990
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
Tabor,S;Richardson,CC
通讯作者:
Richardson,CC