Expression of estrogen-responsive finger protein (Efp) is associated with advanced disease in human epithelial ovarian cancer
Expression of estrogen-responsive finger protein (Efp) is associated with advanced disease in human epithelial ovarian cancer
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DOI：
10.1016/j.ygyno.2005.07.103
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发表时间：
2005-12-01
影响因子：
4.7
通讯作者：
Yaegashi, N
中科院分区：
文献类型：
--
作者：
Sakuma, M;Akahira, J;Yaegashi, N
目标。雌激素应答环指蛋白（estrogen-responsive ring finger protein, Efp）基因是雌激素受体（estrogen receptor， ER）靶基因之一，被认为是雌激素依赖性细胞增殖的关键基因。为了了解雌激素对卵巢癌的作用，我们研究了上皮性卵巢癌中Efp与er表达的关系以及Efp表达与临床病理参数的相关性。采用免疫印迹法检测12株卵巢癌细胞株中Efp、ER α和ER β蛋白的表达。采用定量RT-PCR方法检测12株卵巢癌细胞系中Up mRNA的表达情况。对100例确诊为上皮性卵巢癌的手术标本进行Efp、ER α和ER β抗体免疫组织化学检测。12株细胞系中有8株检测到Up蛋白。在Up蛋白阳性细胞株中，Up mRNA的表达量高于阴性细胞株（P = 0.021）。所有的Up蛋白阳性细胞系同时表达ER α或ER β蛋白。通过免疫组化染色，100例卵巢癌标本中63例检测到Efp免疫反应性，癌细胞细胞质中有阳性信号。Efp与ER α、ER β免疫反应性呈显著相关（Efp与ER α， P = 0.022； Efp与ER β， P = 0.032）。在浆液性腺癌（P = 0.010）和晚期疾病（P = 0.026）亚组中，Efp的表达显著升高。未发现Up免疫反应性与总生存率有显著关系。结论。在人上皮性卵巢癌中检测到Efp的表达，Efp的高表达与疾病晚期和浆液性腺癌以及er状态相关。(c) 2005爱思唯尔公司版权所有。
Objective. The estrogen-responsive ring finger protein (Efp) gene, one of estrogen receptor (ER) target genes, is considered to be essential for estrogen-dependent cell proliferation. To understand the estrogenic action on ovarian cancer, we studied the relationships between Efp and ERs expressions and the correlations of Efp expression with clinicopathological parameters in epithelial ovarian cancer.Methods. The protein expressions for Efp, ER alpha and ER beta were examined by immunoblotting in 12 ovarian cancer cell lines. Up mRNA expressions were evaluated by quantitative RT-PCR in 12 ovarian cancer cell lines. A total of 100 surgical specimens diagnosed as epithelial ovarian cancer were examined immunohistochemically using antibodies for Efp, ER alpha and ER beta.Results. Up protein was detected in 8 out of 12 cell lines. In Up protein-positive cell lines, Up mRNA was expressed higher than that in negative (P = 0.021). All of the Up protein-positive cell lines simultaneously expressed either ER alpha or ER beta protein. By immunohistochemical staining, Efp immunoreactivity was detected in 63 out of 100 ovarian cancer specimens and positive signals were in the cytoplasm of carcinoma cells. There were significant correlations between Efp and ER alpha, ER beta immunoreactivity (Efp and ER alpha, P = 0.022; Efp and ER beta, P = 0.032). Efp expression was significantly higher in a subgroup with serous adenocarcinoma (P = 0.010) and with advanced disease (P = 0.026). No significant relationship was detected between Up immunoreactivity and overall survival. Conclusion. The expression of Efp was detected in human epithelial ovarian cancer and high expression of Efp was correlated with advanced disease and serous adenocarcinoma, and ERs status. (c) 2005 Elsevier Inc. All rights reserved.