Flickering calcium microdomains signal turning of migrating cells.
Flickering calcium microdomains signal turning of migrating cells.
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DOI：
10.1139/y09-118
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发表时间：
2010-02
影响因子：
2.1
通讯作者：
Chao-liang Wei;Xianhua Wang;Min Chen;K. Ouyang;M. Zheng;Heping Cheng
中科院分区：
文献类型：
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作者：
Chao-liang Wei;Xianhua Wang;Min Chen;K. Ouyang;M. Zheng;Heping Cheng
众所周知，极化迁移细胞表现出稳定且短暂的细胞内钙浓度 ([Ca2+]i) 梯度，从前到后逐渐增加，这被认为是导致后回缩的原因。出现的悖论是，在细胞迁移过程中，特别是在细胞转向的决策过程中，细胞前端的钙如何催化关键的高阈值钙依赖性过程。在这篇简短的综述中，我们讨论了最近在迁移成纤维细胞前端发现的闪烁高 [Ca2+]i 微区（“钙闪烁”）及其在转导局部膜机械应力（通过 TRPM7，一种拉伸激活的钙渗透瞬时受体电位通道）和趋化剂引发的信号（通过内质中的 2 型肌醇 1,4,5-三磷酸受体）中的常见作用。网状结构）。此外，我们提出了一种新的钙闪烁活动模型，作为引导迁移细胞转动的机制。
It has been well-established that polarized migrating cells exhibit a stable and transient gradient of intracellular calcium concentration ([Ca2+]i), increasing from front-to-rear, that is thought to be responsible for rear retraction. The paradox that arises is how calcium at the front of a cell catalyzes critical high-threshold calcium-dependent processes during cell migration and particularly in decision-making for a cell to turn. In this brief review, we discuss the recent discovery of flickering high-[Ca2+]i microdomains ("calcium flickers") at the front of migrating fibroblasts and their common role in transducing local membrane mechanical stress (via TRPM7, a stretch-activated calcium-permeating transient receptor potential channel) and chemoattractant-elicited signals (via type 2 inositol 1,4,5-trisphosphate receptor in the endoplasmic reticulum). Furthermore, we present a new model for patterned calcium flicker activity as the mechanism for steering the turning of a migrating cell.