DNA analysis of HLA-DR, DQ, and DP alleles in children with polyarticular juvenile rheumatoid arthritis.
DNA analysis of HLA-DR, DQ, and DP alleles in children with polyarticular juvenile rheumatoid arthritis.
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发表时间：
1992-10
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通讯作者：
K. Barron;E. Silverman;J. C. Gonzales;D. Owerbach;J. Reveille
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作者：
K. Barron;E. Silverman;J. C. Gonzales;D. Owerbach;J. Reveille
采用限制性片段长度多态性分析和聚合酶链反应扩增基因组DNA的寡核苷酸探针杂交技术，对94例多关节型幼年类风湿关节炎（JRA）白人儿童[13例类风湿因子（RF）+，81例RF-]和100例健康对照者的HLA-DR、DQ和DP等位基因进行了检测。HLA-DRw 8、DQw 4、DQA 1 *0401、DQB 1 *0402在RF血清阴性疾病患者中的频率增加，在发病年龄较小（< 5岁）的患者中频率最高。这些结果与我们在少关节型JRA儿童中观察到的结果相似，尤其是那些发病年龄较小的儿童。DPB 1 *0301在RF-组中的频率也增加，特别是抗核抗体血清阴性的患者。与在少关节型JRA患者中观察到的情况相反，DPB 1 *0201的频率在任何多关节型JRA患者组中均未增加。这些数据表明，多关节型JRA与少关节型JRA有许多共同的遗传特征。
HLA-DR, DQ and DP alleles were determined by restriction fragment length polymorphism analysis and oligonucleotide probe hybridization of polymerase chain reaction amplified genomic DNA in 94 Caucasian children with polyarticular juvenile rheumatoid arthritis (JRA) [13 rheumatoid factor (RF)+ and 81 RF-] and 100 healthy controls. HLA-DRw8, DQw4, DQA1*0401, DQB1*0402 were increased in frequency in those patients with RF seronegative disease, with highest frequencies seen in patients with young age at onset (< 5 years of age). These findings were similar to what we observed in children with pauciarticular JRA, especially those with young age at onset. DPB1*0301 was also found in increased frequency in the RF- group, and in particular those seronegative for antinuclear antibody. In contrast to what is observed in patients with pauciarticular JRA, the frequency of DPB1*0201 was not increased in any polyarticular JRA patient group. These data suggest that polyarticular JRA shares many genetic features with pauciarticular JRA.