The U8 snoRNA gene family: identification and characterization of distinct, functional U8 genes in Xenopus
The U8 snoRNA gene family: identification and characterization of distinct, functional U8 genes in Xenopus
复制标题
DOI：
10.1016/s0378-1119(01)00596-0
复制
发表时间：
2001-08-22
期刊：
影响因子：
3.5
通讯作者：
McDowell, K
中科院分区：
文献类型：
--
作者：
Peculis, BA;DeGregorio, S;McDowell, K
U8 snoRNA是一种小核仁核糖核蛋白（U8 snoRNP）的RNA组分，是积累成熟5.8S和28S rRNA（核糖体大亚基的组分）所必需的。我们已经确定了两个假定的U8基因在非洲爪蟾。这两个基因的编码区的序列分析表明，这两个不同的几个位置，从以前的特点U8 RNA，这两个不同。这些基因的功能分析表明，这两个在体内转录，产生稳定的U8转录本，并能够促进前rRNA在体内加工。这些数据表明，在非洲爪蟾中的U8 snoRNA基因之间存在天然序列变异。这三个非洲爪蟾U8序列与先前描述的哺乳动物U8同源物在小鼠，大鼠和人类的比对提供了关于进化上保守的序列和U8 RNA的结构元件的信息。识别和。对非洲爪蟾中这些天然存在的变体的功能表征有助于鉴定U8 RNA中可能对功能至关重要的区域。出版社：Elsevier Science B.V.
U8 snoRNA is the RNA component of a small nucleolar ribonucleoprotein (U8 snoRNP) required for accumulation of mature 5.8S and 28S rRNAs, components of the large ribosomal subunit. We have identified two putative U8 genes in Xenopus laevis. Sequence analysis of the coding regions of these two genes indicate that both differ at several positions from the previously characterized U8 RNA and that the two differ from each other. Functional analysis of these genes indicates that both are transcribed in vivo, produce stable U8 transcripts, and are capable of facilitating pre-rRNA processing in vivo. These data demonstrate that natural sequence variation exists among the U8 snoRNA genes in Xenopus. Alignment of these three Xenopus U8 sequences with the previously described mammalian U8 homologues in mouse, rat and human has provided information about evolutionarily conserved sequence and structural elements in U8 RNA. Identification and. functional characterization of these naturally occurring variants in Xenopus has helped identify regions in U8 RNA that may be critical for function. Published by Elsevier Science B.V.