Specific location of sperm mitochondria in mussel Mytilus galloprovincialis zygotes stained by MitoTracker
Specific location of sperm mitochondria in mussel Mytilus galloprovincialis zygotes stained by MitoTracker
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MitoTracker染色贻贝受精卵精子线粒体的具体位置
DOI：
10.1111/j.1440-169x.2005.00801.x
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发表时间：
2005
期刊：
影响因子：
4.6
通讯作者：
A. Komaru
中科院分区：
文献类型：
--
作者：
M. Obata;A. Komaru
在Mytilidae中，后代的线粒体DNA （mtDNA）遗传自雄性和雌性亲本。精子线粒体只与睾丸结合。这种现象被称为双单亲遗传（DUI）。精子线粒体在发育过程中应该位于原始生殖细胞以维持DUI。然而，精子线粒体定位的机制尚不清楚。为了揭示这一机制，我们用MitoTracker染色的精子对Mytilus galloprovincialis受精卵进行了精子线粒体的定位。受精后，从不同位置进入卵子的精子线粒体停留在精子的入口点。五个精子线粒体位于雄性原核。原核膨胀后，精子线粒体随雄性原核一起迁移到卵子的中心。在卵裂后期，精子线粒体的分布模式分为两种模式。在模式A中，精子线粒体位于卵子的赤道区域。在模式B中，精子线粒体迁移并分成两组染色体。通过对秋水仙碱处理过的卵子的观察，我们认为精子线粒体从受精到卵裂后期的迁移依赖于微管。模式A和模式B之间的差异可能是由于精子线粒体是否在卵裂- 1时由微管迁移引起的。
In Mytilidae, mitochondrial DNA (mtDNA) in the offspring is inherited from male and female parents. Sperm mitochondria are only incorporated into the testes. This phenomenon is called doubly uniparental inheritance (DUI). Sperm mitochondria should locate in the primordial germ cell during development to maintain DUI. However, the mechanism of sperm mitochondria localization is still unknown. To reveal the mechanism, we followed the location of sperm mitochondria in Mytilus galloprovincialis zygotes fertilized with sperm stained by MitoTracker. Just after fertilization, sperm mitochondria, which were found to enter eggs from various sites, remained at sperm entry point. Five sperm mitochondria located at the male pronucleus. After pronuclear expansion, sperm mitochondria migrated to the center of the egg together with the male pronucleus. At anaphase of cleavage‐I, the distribution pattern of sperm mitochondria was divided into two patterns. In pattern A, sperm mitochondria located in the equatorial region of the eggs. In pattern B, sperm mitochondria migrated and divided into two groups with chromosomes. From observations of colchicine‐treated eggs, we suggest that sperm mitochondria migration from fertilization to anaphase of cleavage‐I depends on the microtubules. The difference between pattern A and pattern B may be caused by whether sperm mitochondria migrated or not by the microtubules at cleavage‐I.
影响因子：
3.6
作者：
Sutovsky, P;Navara, CS;Schatten, G
通讯作者：
Schatten, G