Brassinosteroid signaling positively regulates abscisic acid biosynthesis in response to chilling stress in tomato
Brassinosteroid signaling positively regulates abscisic acid biosynthesis in response to chilling stress in tomato
复制标题
油菜素类固醇信号传导积极调节脱落酸生物合成以应对番茄的低温胁迫
DOI：
10.1111/jipb.13356
复制
发表时间：
2023-01-04
影响因子：
11.4
通讯作者：
Xia, Xiaojian
中科院分区：
文献类型：
--
作者：
An, Shengmin;Liu, Yue;Xia, Xiaojian
植物甾醇（BRs）和脱落酸（阿坝）是植物生长和抗逆性的重要调节因子。虽然BRs和阿坝的拮抗作用被提出来确保模式植物生长和防御之间的平衡，但BRs和阿坝之间的串扰在番茄（Solanum lycopersicum），一种温暖的气候园艺作物中，响应于冷，是不清楚的。在这里，我们确定BR生物合成基因DWARF（DWARF）或BR信号传导关键基因BRASSINAZOLE-RESISTANT 1（BZR 1）的过表达通过正调节阿坝生物合成基因9-顺式-环氧胡萝卜素二氧合酶1（NCED 1）的表达来增加阿坝水平以响应低温胁迫。BR诱导的耐冷性主要依赖于阿坝的合成。低温胁迫或高BR水平降低了BR信号负调节因子-油菜素类固醇-不敏感2（BIN 2）的丰度。此外，我们观察到，低温胁迫增加BR水平，并导致BZR 1的积累。BIN 2通过抑制阿坝的合成，负调控BZR 1蛋白的积累和耐冷性。我们的研究结果表明，BR信号通过阿坝生物合成正向调节番茄的耐冷性。这项研究对园艺中的温暖气候作物的生产有影响。
Brassinosteroids (BRs) and abscisic acid (ABA) are essential regulators of plant growth and stress tolerance. Although the antagonistic interaction of BRs and ABA is proposed to ensure the balance between growth and defense in model plants, the crosstalk between BRs and ABA in response to chilling in tomato (Solanum lycopersicum), a warm-climate horticultural crop, is unclear. Here, we determined that overexpression of the BR biosynthesis gene DWARF (DWF) or the key BR signaling gene BRASSINAZOLE-RESISTANT1 (BZR1) increases ABA levels in response to chilling stress via positively regulating the expression of the ABA biosynthesis gene 9-CIS-EPOXYCAROTENOID DIOXYGENASE1 (NCED1). BR-induced chilling tolerance was mostly dependent on ABA biosynthesis. Chilling stress or high BR levels decreased the abundance of BRASSINOSTEROID-INSENSITIVE2 (BIN2), a negative regulator of BR signaling. Moreover, we observed that chilling stress increases BR levels and results in the accumulation of BZR1. BIN2 negatively regulated both the accumulation of BZR1 protein and chilling tolerance by suppressing ABA biosynthesis. Our results demonstrate that BR signaling positively regulates chilling tolerance via ABA biosynthesis in tomato. The study has implications in production of warm-climate crops in horticulture.