CENTRIFUGAL SEPARATION OF CELLS IN SPUTUM SPECIMENS FROM PATIENTS WITH UNDIFFERENTIATED CARCINOMA
CENTRIFUGAL SEPARATION OF CELLS IN SPUTUM SPECIMENS FROM PATIENTS WITH UNDIFFERENTIATED CARCINOMA
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DOI：
10.1002/cyto.990070607
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发表时间：
1986-11-01
期刊：
影响因子：
--
通讯作者：
PRESSMAN, NJ
中科院分区：
文献类型：
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作者：
ALBRIGHT, CD;FROST, JK;PRESSMAN, NJ
采用不连续密度梯度离心技术，在水性Ficoll中分离未分化肺癌患者痰液中的乙醇固定细胞。选择性富集未分化的小细胞（例如，燕麦细胞）或大细胞未分化的癌细胞，同时从含有最大相对频率的癌细胞的样品级分中除去大部分白细胞（80-90%）和巨噬细胞（65-75%）。小细胞癌细胞的最大纯度（0.04%）出现在中等密度（ρ）中。=1.121g/ml）梯度级分，并导致相对于未处理的标本的2.4倍富集。相反，在非常高的密度（ρ）下获得大细胞癌细胞的最大纯度（0.22%）。= 1.172 μ g/ml）梯度洗脱，与未处理的样品相比，富集了1.2倍。显微镜检查巴氏染色标本馏分显示，这些富集，同时保留诊断显着的细胞形态学和着色特征，癌症筛查和诊断所需的。未分化癌细胞的峰纯度范围与来自支气管腺癌和鳞状细胞癌的材料的可比范围显著重叠。
Ethanol-fixed cells in sputum from patients with undifferentiated carcinoma of the lung were separated in aqueous Ficoll using a discontinuous density gradient centrifugation technique. The selective enrichment of small cell undifferentiated (e.g., oat cell) or large cell undifferentiated carcinoma cells was achieved while removing most of the leukocytes (80-90%) and macrophages (65-75%) from specimen fractions containing the greatest relative frequencies of cancer cells. The maximum purity of small cell carcinoma cells (0.04%) occurs in moderate density (.rho. =1.121g/ml) gradient fractions and results in a 2.4-fold enrichment relative to unprocessed specimens. In contrast, the maximum purity of large cell carcinoma cells (0.22%) is obtained in very high density (.rho. = 1.172 g/ml) gradient fractions and results in a 1.2-fold enrichment in comparison with unprocessed specimens. Microscopic examination of Papanicolaou-stained specimen fractions reveals that these enrichments were achieved while retaining diagnostically significant cytomorphologic and tinctorial features necessary for cancer screening and diagnosis. Peak purity ranges of undifferentiated cancer cells significantly overlap comparable ranges for material from bronchogenic adenocarcinoma and squamous cell carcinoma.