Clarification of the Blood Compatibility Mechanism by Controlling the Water Structure at the Blood–Poly(meth)acrylate Interface
Clarification of the Blood Compatibility Mechanism by Controlling the Water Structure at the Blood–Poly(meth)acrylate Interface
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DOI：
10.1163/092050610x517220
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发表时间：
2010-01
期刊：
影响因子：
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通讯作者：
Masaru Tanaka;A. Mochizuki
中科院分区：
文献类型：
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作者：
Masaru Tanaka;A. Mochizuki
在之前的研究中，我们报道了聚（2-甲氧基乙基丙烯酸酯）（PMEA）具有良好的血液相容性，尽管它具有简单的化学结构。从那时起，我们一直在调查其血液相容性的原因。在这篇简短的综述中，我们通过将水合PMEA中的水结构与作为参考聚合物的聚甲基丙烯酸2-羟乙基酯（PHEMA）和聚丙烯酸甲基苯丙酯类似物的水结构进行比较，来考虑这种相容性的原因。PMEA的水合水可分为三种类型；自由水（或称冷冻水）、冻结水（或称中间水）和非冻结水（或称非冻结水）。我们发现水合PMEA具有独特的水结构，在差示扫描量热法（DSC）中观察到水的冷结晶。冷结晶被解释为低温下的冰形成，这是PMEA中冻结水的一个属性。对水合聚乙二醇（PEG）、聚乙烯基甲基醚（PVME）、聚乙烯吡咯烷酮（PVP）、聚（2-甲基丙烯酰氧乙基磷酸胆碱）（PMPC）、聚（四氢糠酰丙烯酸酯）（PTHFA）、新合成的聚（2-（2-乙氧基乙氧基）丙烯酸乙酯）以及各种已知的生物相容性聚合物（蛋白质和多糖）观察到冷结晶峰。另一方面，在水合PHEMA和PMEA类似聚合物中没有观察到水的冷结晶，它们没有表现出良好的血液相容性。基于这些发现，我们假设冷冻结合水是PMEA优异的血液相容性的重要原因，它可以防止生物组分直接接触聚合物表面或聚合物表面的非冷冻水。
In previous studies, we reported that poly(2-methoxyethyl acrylate) (PMEA) exhibited excellent blood compatibility, although it has a simple chemical structure. Since then, we have been investigating the reasons for its blood compatibility. In this short review, we consider the reasons for this compatibility by comparing the structure of water in hydrated PMEA to the water structure of poly(2-hydroxyethyl methacrylate) (PHEMA) and poly(meth)acrylate analogs as reference polymers. The hydrated water in PMEA could be classified into three types; free water (or freezing water), freezing-bound water (or intermediate water), and non-freezing water (or non-freezing-bound water). We found that hydrated PMEA possessed a unique water structure, observed as cold crystallization of water in differential scanning calorimetry (DSC). Cold crystallization is interpreted as ice formation at low temperature, an attribute of freezing-bound water in PMEA. The cold crystallization peak was observed for hydrated poly(ethylene glycol) (PEG), poly(vinyl methyl ether) (PVME), polyvinylpyrrolidone (PVP), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC), poly(tetrahydrofurfuryl acrylate) (PTHFA), and newly synthesized poly(2-(2-ethoxyethoxy)ethyl acrylate), as well as various proteins and polysaccharides, which are well-known biocompatible polymers. On the other hand, cold crystallization of water was not observed in hydrated PHEMA and PMEA analogous polymers, which do not show excellent blood compatibility. Based on these findings, we hypothesized that freezing-bound water, which prevents the biocomponents from directly contacting the polymer surface or non-freezing water on the polymer surface, plays an important role in the excellent blood compatibility of PMEA.