Identification and characterization of the mammalian association and actin-nucleating domains in the Rickettsia conorii autotransporter protein, Sca2.
Identification and characterization of the mammalian association and actin-nucleating domains in the Rickettsia conorii autotransporter protein, Sca2.
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DOI：
10.1111/j.1462-5822.2012.01815.x
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发表时间：
2012-09
影响因子：
3.4
通讯作者：
Martinez JJ
中科院分区：
文献类型：
--
作者：
Cardwell MM;Martinez JJ
斑点热群立克次体感染的建立依赖于这些细菌粘附和侵入宿主内皮的能力。最近的研究将这些过程归因于来自自动转运蛋白表面细胞抗原（sca）家族的少数立克次体表面蛋白。来自康氏立克次体的立克次体自身转运蛋白Sca2已被证明足以介导人内皮细胞的粘附和侵袭，并参与细胞内基于肌动蛋白的运动。在这里，我们确定了一个区域的Sca2能够与哺乳动物细胞表面的相互作用，并表明Sca2的这种功能是独立的，可分离的肌动蛋白成核活性。此外，在R. Conorii感染可竞争性抑制立克次体的侵袭，表明Sca 2在与哺乳动物细胞的初始相互作用中起重要作用。总之，我们的结果表明，在R. Conorii含有不同的功能结构域，其可能参与介导质膜和宿主胞质溶胶处的细胞相互作用。
Establishment of infection by spotted fever group rickettsial species is dependent on the ability of these bacteria to adhere to and invade the host endothelium. Recent studies have attributed these processes to a handful of rickettsial surface proteins from the surface cell antigen (sca) family of autotransporters. A rickettsial autotransporter from Rickettsia conorii, Sca2, has been shown to be sufficient to mediate both adherence and invasion of human endothelial cells and to participate in intracellular actin-based motility. Here we identify a region of Sca2 capable of interacting with the mammalian cell surface and show that this function of Sca2 is independent and separable from its actin nucleation activity. Furthermore, pre-incubation of mammalian cells with the Sca2 mammalian association region prior to R. conorii infection can competitively inhibit rickettsial invasion, suggesting that Sca2 plays an important role in the initial interaction with mammalian cells. Together our results demonstrate that the Sca2 autotransporter protein in R. conorii contains distinct functional domains that likely are involved in mediating cellular interactions at the plasma membrane and the host cytosol.
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影响因子：
3.7
作者：
Gillespie, Joseph J.;Williams, Kelly;Shukla, Maulik;Snyder, Eric E.;Nordberg, Eric K.;Ceraul, Shane M.;Dharmanolla, Chitti;Rainey, Daphne;Soneja, Jeetendra;Shallom, Joshua M.;Vishnubhat, Nataraj Dongre;Wattam, Rebecca;Purkayastha, Anjan;Czar, Michael;Crasta, Oswald;Setubal, Joao C.;Azad, Abdu F.;Sobral, Bruno S.
通讯作者：
Sobral, Bruno S.
DOI：
10.4269/ajtmh.1985.34.361
发表时间：
1985-01-01
影响因子：
3.3
作者：
WALKER, DH;GEAR, JHS
通讯作者：
GEAR, JHS
影响因子：
4.4
作者：
Baldridge, GD;Burkhardt, NY;Munderloh, UG
通讯作者：
Munderloh, UG
影响因子：
21.3
作者：
Haglund CM;Choe JE;Skau CT;Kovar DR;Welch MD
通讯作者：
Welch MD
影响因子：
3.1
作者：
Kleba, Betsy;Clark, Tina R.;Hackstadt, Ted
通讯作者：
Hackstadt, Ted