Renal renin, angiotensinogen, and ANG I-converting-enzyme gene expression: influence of dietary protein.
Renal renin, angiotensinogen, and ANG I-converting-enzyme gene expression: influence of dietary protein.
复制标题
DOI：
10.1152/ajprenal.1993.264.6.f981
复制
发表时间：
1993-06
期刊：
影响因子：
--
通讯作者：
M. Martínez‐Maldonado;J. Benabe;Josiah N. Wilcox;Susheng Wang;Chuying Luo
中科院分区：
文献类型：
--
作者：
M. Martínez‐Maldonado;J. Benabe;Josiah N. Wilcox;Susheng Wang;Chuying Luo
低蛋白（LP）喂养（6%）大鼠导致肾脏血流动力学改变，这种改变被转换酶和非肽AT1抑制剂所消除，提示肾内血管紧张素II （ANG II）的作用。在完整和部分肾切除的大鼠中，膳食蛋白质可刺激肾素mRNA的表达。在本研究中，通过原位杂交和Northern blot分析，LP增加肾素免疫反应性和肾素mRNA；全肾血管紧张素原mRNA表达无明显变化。与正常蛋白相比，LP组全肾、皮质和髓质ANG i转换酶（ACE） mRNA也升高。尽管LP肾脏中蛋白质合成率（RNA-to-DNA比率）降低，但肾脏中甘油醛-3-磷酸脱氢酶mRNA的表达并未发生变化。这些研究首次表明，低脂饮食增加了完整大鼠肾肾素和ACE mRNA的表达；这些可能是造成LP肾血流动力学改变的原因。
Low-protein (LP) feeding (6%) of rats results in renal hemodynamic changes that are abolished by converting enzyme and nonpeptide AT1 inhibitors, suggesting a role for intrarenal angiotensin II (ANG II). Dietary protein is a stimulus for the expression of renal renin mRNA in intact and partially nephrectomized rats. In the present study, LP increased renal renin immunoreactivity and renin mRNA as assessed by in situ hybridization and Northern blot analysis; whole kidney expression of angiotensinogen mRNA was unaltered. Whole kidney, cortical, and medullary ANG I-converting-enzyme (ACE) mRNA was also increased in LP vs. normal protein. The changes occurred despite a reduced protein synthetic rate (RNA-to-DNA ratio) in the kidney of LP, which did not change expression of renal glyceraldehyde-3-phosphate dehydrogenase mRNA. These studies show for the first time that LP diet increases the expression of renal renin and ACE mRNA in intact rats; these may be responsible for the renal hemodynamic changes of LP.