HEPATIC ACAT ACTIVITY IN AFRICAN-GREEN MONKEYS IS HIGHLY CORRELATED TO PLASMA LDL CHOLESTERYL ESTER ENRICHMENT AND CORONARY-ARTERY ATHEROSCLEROSIS
HEPATIC ACAT ACTIVITY IN AFRICAN-GREEN MONKEYS IS HIGHLY CORRELATED TO PLASMA LDL CHOLESTERYL ESTER ENRICHMENT AND CORONARY-ARTERY ATHEROSCLEROSIS
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DOI：
10.1161/01.atv.12.11.1274
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发表时间：
1992-11-01
期刊：
影响因子：
--
通讯作者：
RUDEL, LL
中科院分区：
文献类型：
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作者：
CARR, TP;PARKS, JS;RUDEL, LL
以往的研究和本研究对非洲绿猴的研究表明，血浆低密度脂蛋白（LDL）大小与冠状动脉粥样硬化（CAA）程度之间存在很强的正相关关系。LDL大小的增加主要是由于颗粒核心内胆固醇油酸酯分子的积累，这表明许多胆固醇酯是组织来源，即来自酰基辅酶A：胆固醇酰基转移酶（ACAT）反应，而不是卵磷脂：胆固醇酰基转移酶（LCAT）反应。目前的研究是为了验证这样一种假设，即肝脏中的ACAT是血浆LDL颗粒中胆固醇油酸酯分子数量增加的来源，这似乎增加了LDL的动脉粥样硬化潜力。在肝脏灌注、ACAT测定和CAA评估之前，给猴子喂食富含脂肪（猪油、红花油或鱼油）和胆固醇的饲料3-6年。肝脏ACAT活性与肝脏胆固醇酯分泌呈正相关(r=0.61, p
Previous studies and this study of African green monkeys show a strong positive correlation between plasma low density lipoprotein (LDL) size and the extent of coronary artery atherosclerosis (CAA). Increased LDL size was principally due to the accumulation of cholesteryl oleate molecules within the particle core, suggesting that many of these cholesteryl esters were of tissue origin, i.e., from the acyl-coenzyme A:cholesterol acyltransferase (ACAT) reaction instead of the lecithin:cholesterol acyltransferase (LCAT) reaction. The current study was conducted to test the hypothesis that ACAT in the liver is the source of the increased numbers of cholesteryl oleate molecules in plasma LDL particles that appear to increase the atherogenic potential of LDL. Monkeys were fed diets rich in fat (lard, safflower oil, or fish oil) and cholesterol for 3-6 years before liver perfusion, ACAT assay, and evaluation of CAA. Hepatic ACAT activity was positively correlated with hepatic cholesteryl ester secretion (r=0.61, p