An ultrasensitive new DNA microarray chip provides gene expression profiles for preoperative esophageal cancer biopsies without RNA amplification
An ultrasensitive new DNA microarray chip provides gene expression profiles for preoperative esophageal cancer biopsies without RNA amplification
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DOI：
10.1159/000136156
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发表时间：
2007-01-01
期刊：
影响因子：
3.5
通讯作者：
Shimada, Yutaka
中科院分区：
文献类型：
--
作者：
Ito, Tetsuo;Tanaka, Eiji;Shimada, Yutaka
目的：使用预处理活检的基因表达谱由于样本量小而受到限制。本研究评估了一种具有独特阵列结构的超灵敏新型DNA微阵列芯片在食管癌术前活检临床诊断中的应用价值。方法：对56例食管切除术患者和48例术前内镜检查患者的配对癌和正常食管上皮组织进行研究。在鉴定样本恶性状态的参考DNA芯片选择的2个特征基因集中，选择20个特征基因用于开发新的DNA芯片。将新DNA芯片与每片0.1 μ g的总RNA杂交，不进行RNA扩增。结果：在42例活组织检查中，新DNA芯片的20个特征基因RRM-2、XRCC-3鉴别癌与非癌的准确率为95.2%（敏感性95.7%，特异性94.7%）。受试者工作特征（ROC）曲线分析显示，预测的ROC曲线下面积为0.966。结论：应用超灵敏DNA芯片，无需RNA扩增，术前活检的基因表达谱能准确诊断食管癌。这种新的DNA芯片技术可能有助于进一步开发针对各种癌症患者的定制治疗策略。版权所有(c) 2008 S. Karger AG，巴塞尔。
Objectives: Gene expression profiling using pretreatment biopsies has been limited due to their small sample sizes. This study evaluated the usefulness of an ultrasensitive new DNA microarray chip, which has a unique array structure, for the clinical diagnosis of esophageal cancer using preoperative biopsies. Methods: Paired cancer and normal esophageal epithelial tissues from 56 patients who underwent esophagectomy and from 48 patients who underwent preoperative endoscopy were studied. Among 2 feature gene sets selected by a reference DNA chip discriminating malignant status of samples, 20 feature genes were selected for the development of the new DNA chip. The new DNA chip was hybridized with 0.1 mu g of total RNA per slide without RNA amplification. Results: Twenty feature genes, including RRM-2 and XRCC-3, for the new DNA chip could discriminate cancer from noncancer at a 95.2% rate of accuracy in 42 biopsies ( sensitivity 95.7%, specificity 94.7%). A receiver operating characteristic (ROC) curve analysis showed that the area under ROC curve for the prediction was 0.966. Conclusions: The gene expression profiles from the preoperative biopsies could diagnose esophageal cancer accurately, using the ultrasensitive DNA chip without RNA amplification. This new DNA chip technology might contribute further to the development of customized therapeutic strategies for various cancer patients. Copyright (c) 2008 S. Karger AG, Basel.