Expression of small intestinal and colonic phenotypes in complete intestinal metaplasia of the human stomach
Expression of small intestinal and colonic phenotypes in complete intestinal metaplasia of the human stomach
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DOI：
10.1007/s00428-005-0040-1
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发表时间：
2005-08
期刊：
影响因子：
3.5
通讯作者：
Harunari Tanaka;T. Tsukamoto;T. Mizoshita;K. Inada;N. Ogasawara;Xueyuan Cao;S. Kato;T. Joh;M. Tatematsu
中科院分区：
文献类型：
--
作者：
Harunari Tanaka;T. Tsukamoto;T. Mizoshita;K. Inada;N. Ogasawara;Xueyuan Cao;S. Kato;T. Joh;M. Tatematsu
据报道，不完全肠上皮化生（IM）是人类胃癌发生的一个危险因素，通常以产生磺粘蛋白为特征，因此被认为是结肠型。为了阐明其潜在的机制，我们在此通过免疫组织化学和实时逆转录聚合酶链反应在单个孤立腺体水平上研究了IM中结肠和小肠表型的比例。碳酸酐酶1（CA 1）是结肠上皮细胞的特异性标志物，而蔗糖酶则是小肠吸收细胞的特异性标志物。在来自癌症患者的切除的幽门粘膜中，分别有139个（23.5%）和452个（76.5%）IM腺体被判定为CA 1阳性和CA 1阴性。CA 1阳性的IMs中MUC 5AC的平均得分显著低于CA 1阴性的对应组织（P<0.0001），而蔗糖酶的情况则相反（P<0.0001）。高铁二胺-阿尔新蓝染色显示CA 1表达与I型完全性IM一致。CA 1 mRNA的表达与蔗糖酶-异麦芽糖酶的表达密切相关，与MUC 5AC的表达呈负相关。总之，CA 1可以与小肠蛋白如蔗糖酶共定位，但很少与胃粘蛋白MUC 5AC共定位。其表达需要进一步研究，重点是胃和肠转录因子的刺激和/或抑制机制。
The incomplete intestinal metaplasia (IM) that is reported to be a risk factor for gastric carcinogenesis in man usually features sulfomucin production and thus is considered of colonic type. To cast light on the underlying mechanisms, we here examined the proportions of colonic and small intestinal phenotypes in IM by immunohistochemistry and real-time reverse transcription–polymerase chain reaction at the single isolated gland level. Carbonic anhydrase 1 (CA1) is a specific marker of colonic epithelial cells, whereas sucrase is specific to absorptive cells of the small intestine. Totals of 139 (23.5%) and 452 (76.5%) IM glands were judged to be CA1 positive and CA1 negative, respectively, in resected pyloric mucosa from cancer patients. The average score for MUC5AC in CA1-positive IMs was significantly lower than in CA1-negative counterpart tissue (P<0.0001), whereas the opposite was the case for sucrase (P<0.0001). High iron diamine–Alcian blue staining revealed CA1 expression to coincide with type I complete IM. The expression of CA1 mRNA strongly correlated with that of sucrase–isomaltase, and inversely with that of MUC5AC in isolated IM glands. In conclusion, CA1 could be colocalized with small intestinal proteins such as sucrase, but only rarely with the gastric mucin, MUC5AC. Its expression warrants further study, with the focus on stimulation and/or suppression mechanisms by gastric and intestinal transcription factors.