In-vitro anti-inflammatory activity of Pinus sylvestris and Plantago lanceolata extracts:: effect on inducible NOS, COX-1, COX-2 and their products in J774A.1 murine macrophages
In-vitro anti-inflammatory activity of Pinus sylvestris and Plantago lanceolata extracts:: effect on inducible NOS, COX-1, COX-2 and their products in J774A.1 murine macrophages
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DOI：
10.1211/0022357055605
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发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Perez-Fernandez, R
中科院分区：
文献类型：
--
作者：
Vigo, E;Cepeda, A;Perez-Fernandez, R
植物物种樟子松的提取物。和轮叶车前。在传统医学中用于治疗某些呼吸道疾病，但对其确切效果和作用机制知之甚少。在这项研究中，我们研究了这些植物提取物对一氧化氮（NO）和前列腺素E-2，NO合成酶（NOS）11型，环氧合酶-1（考克斯-1）和考克斯-2 mRNA表达的小鼠巨噬细胞系J774 A.1的生产的影响。我们发现樟子松和车前提取物以浓度依赖的方式抑制该细胞系中NO的产生，MTT法检测没有明显的细胞毒性作用。在所有使用的剂量下的车前提取物和在高剂量下的樟子松提取物显示出对由NO供体PAPA-NONOate释放的NO自由基的显著清除。我们的数据还表明，这些提取物的预处理显着抑制诱导型一氧化氮合酶（iNOS）mRNA的生产在这个细胞系，而不影响考克斯-1 mRNA的表达。经提取物预处理后，脂多糖/干扰素-γ诱导的考克斯-2 mRNA水平和PGE（2）水平未发生改变。因此，我们的研究结果表明，樟子松和车前提取物的抗炎特性可能反映了NO的产生减少，可能是由于对iNOS基因表达或NO清除活性的抑制作用。
Extracts of the plant species Pinus sylvestris L. and Plantago lanceolata L. have been used in traditional medicine for the treatment of certain respiratory diseases, but little is known about their precise effects and mechanisms of action. In this study, we investigated the effect of these plant extracts on the production of nitric oxide (NO) and prostaglandin E-2, NO synthase (NOS) type 11, cyclooxygenase-1 (COX-1) and COX-2 mRNA expression in the murine macrophage cell line J774A.1. We found that Pinus sylvestris and Plantago lanceolata extracts inhibited NO production in a concentration-dependent manner in this cell line, without obvious cytotoxic effects as tested by MTT assay. The Plantago lanceolata extract at all doses used, and the Pinus sylvestris extract at high doses, showed significant scavenging of NO radicals released by the NO donor PAPA-NONOate. Our data also show that pre-treatment with these extracts significantly inhibits inducible NOS (iNOS) mRNA production in this cell line, without affecting COX-1 mRNA expression. COX-2 mRNA levels and PGE(2) levels induced by lipopolysaccharide/interferon-gamma were not modified upon pre-treatment with the extracts. Thus, our results suggest that the anti-inflammatory properties of Pinus sylvestris and Plantago lanceolata extracts may reflect decreased NO production, possibly due to inhibitory effects on iNOS gene expression or to NO-scavenging activity.