Insect ryanodine receptor: distinct but coupled insecticide binding sites for [N-C(3)H(3)]chlorantraniliprole, flubendiamide, and [(3)H]ryanodine.
Insect ryanodine receptor: distinct but coupled insecticide binding sites for [N-C(3)H(3)]chlorantraniliprole, flubendiamide, and [(3)H]ryanodine.
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DOI：
10.1021/tx300326m
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发表时间：
2012-08-20
影响因子：
4.1
通讯作者：
Casida JE
中科院分区：
文献类型：
--
作者：
Isaacs AK;Qi S;Sarpong R;Casida JE
合成了具有高比活性的放射性标记邻氨基苯二胺杀虫剂[N-C3H3]氯坦尼丙醇，并与邻苯二甲胺杀虫剂氟苯二胺和[~3H]ryanodine进行了放射性配基结合研究，首次直接证明了主要的邻氨基苯甲胺和邻苯二甲胺类杀虫剂结合在不同的变构偶联部位，即存在三个不同的钙释放通道靶标进行杀虫作用。
Radiolabeled anthranilic diamide insecticide [N-C3H3]chlorantraniliprole was synthesized at high specific activity and compared with phthalic diamide insecticide flubendiamide and [3H]ryanodine in radioligand binding studies with house fly muscle membranes to provide the first direct evidence with a native insect ryanodine receptor that the major anthranilic and phthalic diamide insecticides bind at different allosterically coupled sites, i.e. there are three distinct Ca2+-release channel targets for insecticide action.