Propionibacterium acnes induces an adjuvant effect in B-1 cells and affects their phagocyte differentiation via a TLR2-mediated mechanism
Propionibacterium acnes induces an adjuvant effect in B-1 cells and affects their phagocyte differentiation via a TLR2-mediated mechanism
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DOI：
10.1016/j.imbio.2016.03.003
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发表时间：
2016-09-01
期刊：
影响因子：
2.8
通讯作者：
Longo-Maugeri, Ieda Maria
中科院分区：
文献类型：
--
作者：
Gambero, Monica;Teixeira, Daniela;Longo-Maugeri, Ieda Maria
B-1淋巴细胞大量存在于小鼠腹腔中，巨噬细胞也是如此，并且负责天然IgM的产生。这些淋巴细胞迁移到炎性病灶，也参与先天免疫。B-1细胞能分化为吞噬细胞（B-1CDP），其特征是表达F4/80，吞噬活性增强，而B-1细胞对抗原和佐剂的反应性很差。已显示痤疮丙酸杆菌悬浮液在巨噬细胞和B-2淋巴细胞中诱导免疫调节作用。我们最近证明，这种细菌有能力增加B-1细胞群体在体外和体内。痤疮丙酸杆菌诱导B-1和B-1CDP细胞分化，增加TLR 2、TLR 4和TLR 9的表达，增加CD 80、CD 86和CD 40的表达，由于痤疮丙酸杆菌对TLR表达有调节作用，因此本研究在体外和体内研究了TLR 2和TLR 4在B-1细胞群中的作用，包括B-1CDP分化和吞噬活性。有趣的是，我们已经证明TLR 2信号传导可能参与痤疮丙酸杆菌诱导的B-1细胞群体的增加。此外，B-1 CDP的早期分化也依赖于TLR 2。还观察到TLR信号还干扰B-1细胞及其吞噬细胞的吞噬能力。根据这些数据，很明显，痤疮丙酸杆菌通过诱导B-1细胞分化为B-1CDP细胞并在体内和体外调节其吞噬功能来促进B-1细胞中的重要佐剂作用。此外，大多数这些作用主要通过TLR 2介导。这些数据加强了这样的细菌悬浮液具有强大的佐剂特性的发现。B-1细胞对外源性刺激的反应表明这些细胞对先天性免疫反应是重要的。(C)2016年爱思唯尔有限公司。All rights reserved.
B-1 lymphocytes are present in large numbers in the mouse peritoneal cavity, as are macrophages, and are responsible for natural IgM production. These lymphocytes migrate to inflammatory foci and are also involved in innate immunity. It was also demonstrated that B-1 cells are able to differentiated into phagocytes (B-1CDP), which is characterized by expression of F4/80 and increased phagocytic activity.B-1 cell responses to antigens and adjuvants are poorly characterized. It has been shown that Propionibacterium acnes suspensions induce immunomodulatory effects in both macrophages and B-2 lymphocytes. We recently demonstrated that this bacterium has the ability to increase B-1 cell populations both in vitro and in vivo. P. acnes induces B-1CDP differentiation, increases the expression of TLR2, TLR4 and TLR9 and augments the expression of CD80, CD86 and CD40 in B-1 and B-1CDP cells.Because P. acnes has been shown to modulate TLR expression, in this study, we investigated the role of TLR2 and TLR4 in B-1 cell population, including B-1CDP differentiation and phagocytic activity in vitro and in vivo. Interestingly, we have demonstrated that TLR2 signaling could be involved in the increase in the B-1 cell population induced by P. acnes. Furthermore, the early differentiation of B-1CDP is also dependent of TLR2. It was also observed that TLR signals also interfere in the phagocytic ability of B-1 cells and their phagocytes. According to these data, it is clear that P. acnes promotes an important adjuvant effect in B-1 cells by inducing them to differentiate into B-1CDP cells and modulates their phagocytic functions both in vivo and in vitro. Moreover, most of these effects are mediated primarily via TLR2. These data reinforce the findings that such bacterial suspensions have powerful adjuvant properties. The responses of B-1 cells to exogenous stimulation indicate that these cells are important to the innate immune response. (C) 2016 Elsevier GmbH. All rights reserved.