Highly efficient monolithic silica capillary columns modified with poly(acrylic acid) for hydrophilic interaction chromatography
Highly efficient monolithic silica capillary columns modified with poly(acrylic acid) for hydrophilic interaction chromatography
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DOI：
10.1016/j.chroma.2007.07.012
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发表时间：
2007-09-14
影响因子：
4.1
通讯作者：
Tanaka, Nobuo
中科院分区：
文献类型：
--
作者：
Horie, Kanta;Ikegami, Tohru;Tanaka, Nobuo
采用锚基修饰的熔融石英毛细管，在柱上聚合丙烯酸，制备了亲水作用液相色谱整体柱。该产品保持了高渗透性（K = 5 × 10 ~（-14）m ~（2）），并在最佳线速度（u）下提供了小于10 μ m的板高（H），在u = 6 mm/s时，对于极性溶质（包括核苷和碳水化合物）提供了小于20 μ m的板高（H）。HILIC模式整体硅胶毛细管柱能够在20 MPa或更低的压降下产生10000个理论塔板（N），柱死时间（to）为20 s。总性能远高于目前可用的常规颗粒填充HILIC柱。通过毛细管LC-电喷雾质谱系统的肽的梯度分离导致在反相模式分离和HILIC模式分离之间非常不同的保留选择性，峰容量为约1.05 μ g/ml。在任一模式下，10 min梯度时间内为100。与改性与聚（丙烯酸）的整体硅胶毛细管柱观察到的高性能表明，HILIC模式可以是一种替代的反相模式的化合物范围很广，特别是对于那些高极性的等度以及梯度洗脱。(c)2007 Elsevier B. V.保留所有权利。
Monolithic silica capillary columns for hydrophilic interaction liquid chromatography (HILIC) were prepared by on-column polymerization of acrylic acid on monolithic silica in a fused silica capillary modified with anchor groups. The products maintained the high permeability (K = 5 x 10(-14) m(2)) and provided a plate height (H) of less than 10 mu m at optimum linear velocity (u) and H below 20 mu m at u = 6 mm/s for polar solutes including nucleosides and carbohydrates. The HILIC mode monolithic silica capillary column was able to produce 10000 theoretical plates (N) with column dead time (to) of 20 s at a pressure drop of 20 MPa or lower. The total performance was much higher than conventional particle-packed HILIC columns currently available. The gradient separations of peptides by a capillary LC-electrospray mass spectrometry system resulted in very different retention selectivity between reversed-phase mode separations and the HILIC mode separations with a peak capacity of ca. 100 in a 10 min gradient time in either mode. The high performance observed with the monolithic silica capillary column modified with poly(acrylic acid) suggests that the HILIC mode can be an alternative to the reversed-phase mode for a wide range of compounds, especially for those of high polarity in isocratic as well as gradient elution. (c) 2007 Elsevier B.V. All rights reserved.