A portable blood plasma clot micro-elastometry device based on resonant acoustic spectroscopy
A portable blood plasma clot micro-elastometry device based on resonant acoustic spectroscopy
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DOI：
10.1063/1.4926543
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发表时间：
2015-07-01
影响因子：
1.6
通讯作者：
Oldenburg, Amy L.
中科院分区：
文献类型：
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作者：
Krebs, C. R.;Li, Ling;Oldenburg, Amy L.
血凝块硬度异常是多种心血管疾病和药物治疗引起的凝血功能障碍的重要指标。在这里，我们提出了一种便携式仪器，用于微升体积血液样本的弹性测量，该仪器基于共振声学光谱原理，其中具有明确尺寸的样本显示出与杨氏模量的平方根成正比的基本纵向共振模式。与商业血栓弹性成像相比，由于共振振动的高信噪比，共振声学方法提供了更高的重复性和准确性。我们回顾了测量原理和磁致微珠力传感器的设计，应用在23 pN和6.7 nN之间，提供了一个宽的弹性模量动态范围（3 Pa-27 kPa），适合测量凝块弹性模量（CEM）。介绍了一种自动化便携式设备CEMport，该设备使用2nm分辨率的位移传感器，其准确度和精度分别为生物凝胶中CEM的3%和2%。重要的是，小型菌株(
Abnormal blood clot stiffness is an important indicator of coagulation disorders arising from a variety of cardiovascular diseases and drug treatments. Here, we present a portable instrument for elastometry of microliter volume blood samples based upon the principle of resonant acoustic spectroscopy, where a sample of well-defined dimensions exhibits a fundamental longitudinal resonance mode proportional to the square root of the Young's modulus. In contrast to commercial thromboelastography, the resonant acoustic method offers improved repeatability and accuracy due to the high signal-to-noise ratio of the resonant vibration. We review the measurement principles and the design of a magnetically actuated microbead force transducer applying between 23 pN and 6.7 nN, providing a wide dynamic range of elastic moduli (3 Pa-27 kPa) appropriate for measurement of clot elastic modulus (CEM). An automated and portable device, the CEMport, is introduced and implemented using a 2 nm resolution displacement sensor with demonstrated accuracy and precision of 3% and 2%, respectively, of CEM in biogels. Importantly, the small strains (