Dimerization of GTR1 regulates their plasma membrane localization
Dimerization of GTR1 regulates their plasma membrane localization
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DOI：
10.1080/15592324.2017.1334749
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发表时间：
2017-01-01
影响因子：
2.9
通讯作者：
Ueda, Minoru
中科院分区：
文献类型：
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作者：
Ishimaru, Yasuhiro;Washiyama, Kento;Ueda, Minoru
硝酸盐转运蛋白1/肽转运蛋白家族（NPF）成员是植物激素等多种化合物的多功能转运蛋白，在植物生长和环境胁迫响应中发挥重要作用。最近，我们发现拟南芥GTR 1（也称为NPF 2.10）除了芥子油苷外，还吸收赤霉酸和茉莉酰-L-异亮氨酸。对于正常的植物生长，GTR 1在基因表达水平上受到调节;然而，目前还不清楚是否也发生翻译后调节。在这里，我们发现，GTR 1的二聚化，可能诱导的Thr残基之间的可能的跨膜区域，调节其质膜定位，导致在非洲爪蟾卵母细胞中的芥子油苷和赤霉酸的运输。这些发现表明多功能转运蛋白的二聚化有助于其在质膜上的活性。
Members of the nitrate transporter 1/peptide transporter family (NPF) are multifunctional transporters of various compounds including plant hormones and play important roles in plant growth and responses to environmental stress. Recently, we found that Arabidopsis GTR1 (also known as NPF2.10) takes up gibberellic acid and jasmonoyl-L-isoleucine in addition to glucosinolates. For normal plant growth, GTR1 is regulated at the gene expression level; however, it is unclear whether post-translational regulation also occurs. Here, we found that dimerization of GTR1, possibly induced by dephosphorylation of the Thr residue located between the possible transmembrane regions, regulates its plasma membrane localization, leading to transport of glucosinolates and gibberellic acid in Xenopus oocytes. These findings suggest that dimerization of multifunctional transporters contributes to their activities at the plasma membrane.