Small interfering RNAs as a tool to assign Rho GTPase exchange-factor function in vivo
Small interfering RNAs as a tool to assign Rho GTPase exchange-factor function in vivo
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DOI：
10.1042/bj20020844
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发表时间：
2002-09-01
影响因子：
4.1
通讯作者：
Mellor, H
中科院分区：
文献类型：
--
作者：
Gampel, A;Mellor, H
Rho GTP酶控制细胞内信号传导途径的复杂网络。虽然已经取得了进展，在确定这些蛋白质的下游信号合作伙伴，Rho上游调控鸟嘌呤核苷酸交换因子（GEFs）的表征已受到阻碍，缺乏合适的研究工具。在这里，我们使用小干扰RNA（siRNA）来检查RhoB GT3的细胞调节，并显示RhoB通过Vav 2交换因子在表皮生长因子受体下游被激活。这些研究表明，siRNA是一个理想的研究工具，在体内分配Rho GEF功能。
Rho GTPases control a complex network of intracellular signalling pathways. Whereas progress has been made in identifying downstream signalling partners for these proteins, the characterization of Rho upstream regulatory guanine-nucleotide exchange factors (GEFs) has been hampered by a lack of suitable research tools. Here we use small interfering RNAs (siRNAs) to examine the cellular regulation of the RhoB GTPase, and show that RhoB is activated downstream of the epidermal-growth-factor receptor through the Vav2 exchange factor. These studies demonstrate that siRNAs are an ideal research tool for the assignment of Rho GEF function in vivo.