EIN2-mediated signaling is involved in pre-invasion defense in Nicotiana benthamiana against potato late blight pathogen, Phytophthora infestans
EIN2-mediated signaling is involved in pre-invasion defense in Nicotiana benthamiana against potato late blight pathogen, Phytophthora infestans
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DOI：
10.1080/15592324.2017.1300733
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发表时间：
2017-04
影响因子：
2.9
通讯作者：
S. Rin;Yuri Mizuno;Y. Shibata;Mayuka Fushimi;S. Katou;I. Sato;S. Chiba;K. Kawakita;D. Takemoto
中科院分区：
文献类型：
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作者：
S. Rin;Yuri Mizuno;Y. Shibata;Mayuka Fushimi;S. Katou;I. Sato;S. Chiba;K. Kawakita;D. Takemoto
摘要 本塞姆氏烟草 ABCG1 和 ABCG2 是 ABC 转运蛋白，可能参与烟草种主要植物抗毒素——辣椒素的输出。虽然这些转运蛋白输出的辣椒二醇在针对致病疫霉的入侵后防御中发挥着重要作用，但它们也输出参与入侵前防御的未鉴定的抗菌化合物。在本研究中，使用 GFP 标记分析了 NbABCG2 (Pabcg2a) 的启动子活性。通过与致病疫霉的 INF1 诱导子共表达，Pabcg2a 控制下的 GFP 表达显着增加。 Pabcg2a 中乙烯响应性 GCC 盒的破坏会损害 INF1 诱导的 Pabcg2a 激活。一致地，致病疫霉的渗透通过 NbEIN2（关键的乙烯信号成分）的基因沉默而增加，这表明乙烯参与了本塞姆氏病菌的入侵前防御。
ABSTRACT Nicotiana benthamiana ABCG1 and ABCG2 are ABC transporters which are probably involved in the export of capsidiol, the major phytoalexin of Nicotiana species. While capsidiol export by these transporters plays an essential role in post-invasion defense against Phytophthora infestans, they also export unidentified antimicrobial compound(s) involved in pre-invasion defense. In this study, promoter activity of NbABCG2 (Pabcg2a) was analyzed using a GFP marker. Expression of GFP under the control of Pabcg2a was significantly increased by co-expression with the INF1 elicitor from P. infestans. Disruption of the ethylene-responsive GCC box in Pabcg2a compromised INF1-induced activation of Pabcg2a. Consistently, penetration by P. infestans was increased by gene-silencing of NbEIN2, the key ethylene-signaling component, suggesting the involvement of ethylene for pre-invasion defense of N. benthamiana.