The transcriptome of sesquiterpenoid biosynthesis in heartwood xylem of Western Australian sandalwood (Santalum spicatum)
The transcriptome of sesquiterpenoid biosynthesis in heartwood xylem of Western Australian sandalwood (Santalum spicatum)
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DOI：
10.1016/j.phytochem.2014.12.009
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发表时间：
2015-05-01
期刊：
影响因子：
3.8
通讯作者：
Bohlmann, Joerg
中科院分区：
文献类型：
--
作者：
Moniodis, Jessie;Jones, Christopher G.;Bohlmann, Joerg
西澳大利亚檀香木(檀香木)芳香的心材油含有倍半萜烯烃和醇的混合物，包括不同水平的珍贵倍半萜醇、α-和β-檀香醇，以及通常高水平的E，E-法尼醇。转录组分析揭示了这种具有商业价值的檀香树种中倍半萜生物合成途径的几乎完整的一套基因的序列。转录组序列是从富含法尼醇的个体树的心材木质部组织中产生的。从12,537个重叠群的组装中，鉴定出7个不同的萜烯合成酶(TPSS)、几个细胞色素P450和烯丙基磷酸酶，以及甲氧戊酸和甲基赤藓糖醇磷酸途径的转录本。其中五个S spicatum TPS序列以前是未知的。克隆了SSpiTPS4的全长cDNAs，并将其功能鉴定为多产物倍半松烯B合成酶，补充了已有的檀香烯和双糖醇合成酶的功能特性。虽然SSpiTPS4和以前克隆的檀香TPSS不能解释E，E-法尼醇在刺桐中的普遍存在，但这项工作和以前的工作中确定的基因可以为未来檀香萜类油组分自然变异的研究奠定基础。(C)2014爱思唯尔有限公司。保留所有权利。
The fragrant heartwood oil of West Australian sandalwood (Santalum spicatum) contains a mixture of sesquiterpene olefins and alcohols, including variable levels of the valuable sesquiterpene alcohols, alpha- and beta-santalol, and often high levels of E,E-farnesol. Transcriptome analysis revealed sequences for a nearly complete set of genes of the sesquiterpenoid biosynthetic pathway in this commercially valuable sandalwood species. Transcriptome sequences were produced from heartwood xylem tissue of a farnesol-rich individual tree. From the assembly of 12,537 contigs, seven different terpene synthases (TPSs), several cytochromes P450, and allylic phosphatases were identified, as well as transcripts of the mevalonic acid and methylerythritol phosphate pathways. Five of the S. spicatum TPS sequences were previously unknown. The full-length cDNA of SspiTPS4 was cloned and the enzyme functionally characterized as a multi-product sesquisabinene B synthase, which complements previous characterization of santalene and bisabolol synthases in S. spicatum. While SspiTPS4 and previously cloned sandalwood TPSs do not explain the prevalence of E,E-farnesol in S. spicatum, the genes identified in this and previous work can form a basis for future studies on natural variation of sandalwood terpenoid oil profiles. (C) 2014 Elsevier Ltd. All rights reserved.