Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes
Reciprocal binding of PARP-1 and histone H1 at promoters specifies transcriptional outcomes
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DOI：
10.1126/science.1149250
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发表时间：
2008-02-08
期刊：
影响因子：
56.9
通讯作者：
Kraus, W. Lee
中科院分区：
文献类型：
--
作者：
Krishnakumar, Raga;Gamble, Matthew J.;Kraus, W. Lee
核小体结合蛋白的作用是调节靶基因的启动子染色质结构和转录。我们使用基因组和基因特异性方法来证明两个这样的因子，组蛋白H1和聚（ADP-核糖）聚合酶- 1 (PARP- 1)，在许多RNA聚合酶II转录启动子上表现出染色质结合的互惠模式。在这些启动子上PARP- 1富集，H1缺失。这种结合模式与活跃转录基因有关。此外，我们发现PARP- 1可以将H1从PARP- 1刺激的启动子子集中排除，这表明PARP- 1和H1在核小体结合水平上存在功能相互作用。因此，尽管H1和PARP- 1在体外具有相似的核小体结合特性和对染色质结构的影响，但它们在体内决定基因表达结果方面具有不同的作用。
Nucleosome- binding proteins act to modulate the promoter chromatin architecture and transcription of target genes. We used genomic and gene- specific approaches to show that two such factors, histone H1 and poly( ADP- ribose) polymerase- 1 ( PARP- 1), exhibit a reciprocal pattern of chromatin binding at many RNA polymerase II - transcribed promoters. PARP- 1 was enriched and H1 was depleted at these promoters. This pattern of binding was associated with actively transcribed genes. Furthermore, we showed that PARP- 1 acts to exclude H1 from a subset of PARP- 1 - stimulated promoters, suggesting a functional interplay between PARP- 1 and H1 at the level of nucleosome binding. Thus, although H1 and PARP- 1 have similar nucleosome- binding properties and effects on chromatin structure in vitro, they have distinct roles in determining gene expression outcomes in vivo.