Myo10 is a key regulator of TNT formation in neuronal cells
Myo10 is a key regulator of TNT formation in neuronal cells
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DOI：
10.1242/jcs.129239
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发表时间：
2013-10-01
影响因子：
4
通讯作者：
Zurzolo, Chiara
中科院分区：
文献类型：
--
作者：
Gousset, Karine;Marzo, Ludovica;Zurzolo, Chiara
在多细胞生物体中，细胞间的交流是必不可少的。隧道纳米管(TNTs)是一种新型的细胞间传播机制，可以传输各种信号、细胞器和病原体。在这里，我们研究了非传统分子马达肌球蛋白-X(Myo10)在神经元CAD细胞内功能性TNTs形成中的作用。Myo10蛋白的表达增加了TNTs的数量和小泡在共培养细胞之间的转移。我们还表明，TNT的形成既需要蛋白质的马达结构域，也需要蛋白质的尾部结构域，并确定Myo10尾部内F2叶的F2叶是TNT形成所必需的。综上所述，这些结果表明，在神经细胞中，TNTs可以来自Myo10驱动的背丝足的一个子集，而不依赖于它与整合素和N-钙粘附素的结合。此外，我们的数据强调了在神经细胞和其他细胞类型中存在建立和调节TNTs的不同机制。
Cell-to-cell communication is essential in multicellular organisms. Tunneling nanotubes (TNTs) have emerged as a new type of intercellular spreading mechanism allowing the transport of various signals, organelles and pathogens. Here, we study the role of the unconventional molecular motor myosin-X (Myo10) in the formation of functional TNTs within neuronal CAD cells. Myo10 protein expression increases the number of TNTs and the transfer of vesicles between co-cultured cells. We also show that TNT formation requires both the motor and tail domains of the protein, and identify the F2 lobe of the FERM domain within the Myo10 tail as necessary for TNT formation. Taken together, these results indicate that, in neuronal cells, TNTs can arise from a subset of Myo10-driven dorsal filopodia, independent of its binding to integrins and N-cadherins. In addition our data highlight the existence of different mechanisms for the establishment and regulation of TNTs in neuronal cells and other cell types.