Origin and recovery of colony-forming units in locally curetted bone marrow of mice.
Origin and recovery of colony-forming units in locally curetted bone marrow of mice.
复制标题
局部刮除小鼠骨髓中集落形成单位的起源和恢复。
DOI：
10.1182/blood.v39.3.331.331
复制
发表时间：
1972
期刊：
影响因子：
20.3
通讯作者：
F. Trobaugh
中科院分区：
文献类型：
--
作者：
W. Knospe;W. Knospe;W. Knospe;Stephanie Ann Gregory;Stephanie Ann Gregory;Stephanie Ann Gregory;Salah G. Husseini;Salah G. Husseini;W. Fried;F. Trobaugh;F. Trobaugh
局部刮除骨髓的组织学研究显示一系列的血块形成，血块被原始的网状组织移位，以及造血再生。组织学上窦状血管再生，12wk后恢复不完全，脂肪增多，残留的骨小梁和囊性区域。集落形成单位(CFU)的恢复与造血细胞的恢复平行，到12周后未超过正常的70%。T6、T6标记染色体切除和照射小鼠的移植实验表明，标记染色体的细胞容易迁移到照射后的骨髓中，但在刮除后2wk内被排除在刮除骨髓之外。三周后，刮除标记细胞可以随时进入刮除的骨髓。早期排斥但较晚出现的移植标记细胞与早期不存在和较晚再生的肝窦血管有关。组织学和细胞遗传学研究支持造血依赖于具有适当骨髓微环境的正弦系统的概念。这些研究支持刮除空洞内骨髓再生的局部起源的概念，但并不表明造血组织起源于刮除后存活的少量残留CFU，还是来自多潜能网状细胞。没有证据表明刮除对未刮除的骨髓有刺激作用。
Histologic study of locally curetted bone marrow showed a sequence of blood clot formation, displacement of the clot by a primitive reticular tissue, and hematopoietic regeneration. Histologic regeneration of sinusoidal vessels, and recovery was incomplete 12 wk later with increased fat, residual trabecular bone, and cystic areas. Recovery of colony-forming units (CFU) paralleled hematopoietic cellular recovery and did not exceed levels of 70% of normal by 12 wk postcurettage. Transplantation of cureted and irradiated mice with T 6 T 6 marker chromosomes demonstrated that cells with marker chromosomes migrated readily into irradiated marrow but were excluded from the curetted marrow for 2 wk after curettage. Three weeks after, curettage marker cells gained ready access to curetted marrow. The early exclusion but later appearance of transplanted marker cells was correlated with the early absence and later regeneration of sinusoidal vessels. The histologic and cytogenetic studies support the concept of a dependence of hematopoiesis upon a sinusoidal system with an appropriate marrow microenvironment. These studies support the concept of a local origin of marrow regeneration within curetted cavities but do not indicate whether the hematopoietic tissue originates from small numbers of residual CFU surviving curettage or from multipotential reticular cells. There was no evidence of a stimulatory effect upon uncuretted marrow by the action of curettage.