Interleukin-17F attenuates H2O2-induced cell cycle arrest
Interleukin-17F attenuates H2O2-induced cell cycle arrest
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Interleukin-17F 减弱 H2O2 诱导的细胞周期停滞
DOI：
10.1016/j.cellimm.2013.12.007
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发表时间：
2014-02-01
影响因子：
4.3
通讯作者：
Liu, Weihuang
中科院分区：
文献类型：
--
作者：
Tong, Zan;Yan, Huichao;Liu, Weihuang
白细胞介素 IL-17F 在结肠上皮细胞中表达，在结肠肿瘤发生中表现出多种功能。然而，IL-17F 在结肠癌细胞周期进展中的作用仍不清楚。在这项研究中，我们分析了 IL-17F 对氧化剂诱导的人结肠癌细胞细胞周期转变的影响。用 H2O2 攻击 IL-17F 过表达和野生型 HCT116 细胞。细胞周期分布分析表明，IL-17F 通过抑制 S 到 G2/M 的转变来减弱 H2O2 诱导的 G2/M 期停滞。我们进一步检查了两个关键细胞周期调节因子 p21 和 p27 的表达水平。结果表明IL-17F可以抑制H2O2诱导的p27上调。同时，IL-17F在H2O2处理后可以增加p38的磷酸化。 p27 水平和 p38 活性的调节可能导致 IL-17F 导致的 G2/M 期阻滞受损。总而言之，我们的研究结果扩展了 IL-17F 作为结肠癌发展的重要因素，并为信号通路提供了新的见解。 (C) 2013 Elsevier Inc. 保留所有权利。
Interleukin IL-17F was expressed in colon epithelial cells and showed multiple functions in colon tumorigenesis. However, the role of IL-17F in colon cancer cell cycle progression remains unclear. In this study, we analyzed the effects of IL-17F on oxidant-induced cell cycle shift in human colon cancer cells. IL-17F overexpressing and wildtype HCT116 cells were challenged with H2O2. Cell cycle distribution analysis showed IL-17F attenuated H2O2-induced G2/M phase arrest by inhibiting S to G2/M transition. We further checked expression levels of two critical cell cycle regulators p21 and p27. The results showed that IL-17F could inhibit H2O2 induced p27 up-regulation. Meanwhile, IL-17F could increase the phosphorylation of p38 after H2O2 treatment. The regulations of p27 level and p38 activity may contribute to the impaired G2/M phase arrest by IL-17F. Taken together, our findings extend IL-17F as an important factor in colon cancer development and provide new insight into the signaling pathway. (C) 2013 Elsevier Inc. All rights reserved.