Antitumor actions of cytokines on new human papillary thyroid carcinoma cell lines.
Antitumor actions of cytokines on new human papillary thyroid carcinoma cell lines.
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DOI：
10.1210/jcem.81.7.8675585
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发表时间：
1996-07
期刊：
影响因子：
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通讯作者：
K. Ohta;X. Pang;L. Berg;J. Hershman
中科院分区：
文献类型：
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作者：
K. Ohta;X. Pang;L. Berg;J. Hershman
为了研究细胞因子对人甲状腺乳头状癌（PTC）细胞生长的体外影响，我们从不同的患者身上建立了6株新的PTC细胞系，分别为BHP 5、14、15、17、18和19。我们利用BHP细胞、NP细胞（PTC细胞系）和ARO细胞（间变性甲状腺癌细胞系）研究了细胞因子的抗增殖作用。这些细胞分别用不同浓度的肿瘤坏死因子- α (TNF α)、干扰素- γ (IFN γ)、白细胞介素-1 β (IL-1 β)和转化生长因子- β 1 （TGF β 1）单独或联合治疗。用[3H]胸苷掺入法和细胞计数法观察细胞增殖情况。在BHP细胞系中，IFN γ、IL-1 β和TGF β 1抑制[3H]胸苷结合并减少细胞数量，但TNF α刺激[3H]胸苷结合。在NP细胞中，每一种细胞因子处理都减少了[3H]胸苷的掺入和细胞数量。相反，ARO细胞的增殖受到TNF α、IL-1 β和TGF β 1的刺激或抵抗。在这些细胞系中，这些细胞因子对[3H]胸苷结合的影响是叠加的。提示IL-1 β和TGF β 1在PTC细胞生长抑制中起关键作用，脱离IL-1 β和TGF β 1的负调控可能是向间变性转变的一步。这些细胞因子的叠加效应表明它们通过不同的途径起作用。
To investigate the in vitro effects of cytokines on the growth of human papillary thyroid carcinoma (PTC) cells, we established six new PTC cell lines, designated BHP 5, 14, 15, 17, 18, and 19, from different patients. We studied the antiproliferative actions of cytokines by using BHP cells, NP cells (PTC cell line), and ARO cells (anaplastic thyroid carcinoma cell line). These cells were treated with various concentrations of tumor necrosis factor-alpha (TNF alpha), interferon-gamma (IFN gamma), interleukin-1 beta (IL-1 beta), and transforming growth factor-beta 1 (TGF beta 1), alone and in combination. Cell proliferation was assessed by [3H]thymidine incorporation and cell number measurement. In BHP cell lines, IFN gamma, IL-1 beta, and TGF beta 1 inhibited [3H]thymidine incorporation and decreased cell number, but TNF alpha stimulated [3H]thymidine incorporation. In NP cells, treatment with each cytokine decreased [3H]thymidine incorporation and cell number. In contrast, the proliferation of ARO cells was either stimulated by or resistant to TNF alpha, IL-1 beta, and TGF beta 1. The effects of these cytokines on [3H]thymidine incorporation were additive in these cell lines. The results suggest that IL-1 beta and TGF beta 1 play a pivotal role in growth inhibition of PTC cells, and the escape from negative control of IL-1 beta and TGF beta 1 may be a step toward anaplastic changes. The additive effects of these cytokines suggest that they act through different pathways.