A simplified micromethod for the determination of carbonic anhydrase and its inhibitors.
A simplified micromethod for the determination of carbonic anhydrase and its inhibitors.
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测定碳酸酐酶及其抑制剂的简化微量方法。
DOI：
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发表时间：
1960
影响因子：
3.5
通讯作者：
T. Maren
中科院分区：
文献类型：
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作者：
T. Maren
本文介绍了一种基于pH变化原理的碳酸酐酶微量定量分析方法。灵敏度是这样的：可以检测到0.05 ml 1：1000稀释的哺乳动物血液的活性。该方法适用于生物材料中碳酸酐酶抑制剂活性的测定。对于乙酰唑胺，灵敏度限值为0.2 µg/g组织。芳香族磺酰胺类化合物抑制碳酸酐酶的性质进行了简要讨论，因为它可能涉及到某些变量的分析方法。
A simple quantitative micromethod for carbonic anhydrase based on the changing pH principle is described. Sensitivity is such that the activity of 0.05 ml of mammalian blood diluted 1:1000 can be detected. The method is suitable for estimating the activity of carbonic anhydrase inhibitors and for their determination in biological material. For acetazolamide, the limit of sensitivity is 0.2 µg/g tissue. The nature of carbonic anhydrase inhibition by aromatic sulfonamides is briefly discussed, as it may relate to certain variables in the analytical method.