Design and testing of aptamer-based electrochemical biosensors for proteins and small molecules
Design and testing of aptamer-based electrochemical biosensors for proteins and small molecules
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DOI：
10.1016/j.bioelechem.2009.04.007
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发表时间：
2009-11-01
影响因子：
5
通讯作者：
Yu, Hua-Zhong
中科院分区：
文献类型：
--
作者：
Cheng, Alan K. H.;Sen, Dipankar;Yu, Hua-Zhong
基于适体的电化学生物传感器的制造作为一种新兴技术，使得小分子和大分子分析物的检测更容易，更快，更适合从基础分析科学到蛋白质生物标志物的早期检测的持续过渡。适体是合成的寡核苷酸，其已经经历了体外选择的迭代循环，以高亲和力结合所选择的特定分析物;利用适体作为识别实体用于开发生物传感器的灵敏而简单的方法是电化学地检测信号。在这篇综述文章中，我们试图总结的国家的最先进的研究进展，已发表在最近几年，特别是，我们专注于电化学生物传感器，将适配体用于传感小的有机分子和蛋白质。基于DNA/RNA修饰电极设计的差异，我们将基于适体的电化学传感器分为三类，分析物检测依赖于：（a）构型变化，即。分析物结合诱导传感器构建体的组装或解离：（B）构象变化。也就是说，分析物结合诱导表面固定的适体链的构象（折叠）改变;和（c）电导率改变，即，分析物结合“打开”表面结合的适体-DNA构建体的导电性。在每一节中，我们将讨论这些新的生物传感器的性能与代表性的例子，在最近的文献报道。(C)2009爱思唯尔有限公司版权所有。
The fabrication of aptamer-based electrochemical biosensors as an emerging technology has made the detection of small and macromolecular analytes easier, faster, and more suited for the ongoing transition from fundamental analytical science to the early detection of protein biomarkers. Aptamers are synthetic oligonucleotides that have undergone iterative rounds of in vitro selection for binding with high affinity to specific analytes of choice; a sensitive yet simple method to utilize aptamers as recognition entities for the development of biosensors is to transduce the signal electrochemically. In this review article, we attempt to summarize the state-of-the-art research progresses that have been published in recent years; in particular, we focus on the electrochemical biosensors that incorporate aptamers for sensing small organic molecules and proteins. Based on differences in the design of the DNA/RNA-modified electrodes, we classify aptamer-based electrochemical sensors into three categories, for which the analyte detection relies on: (a) configurational change, i.e.. the analyte binding induces either an assembly or dissociation of the sensor construct: (b) conformational change. i.e., the analyte binding induces an alteration in the conformation (folding) of the surface immobilized aptamer strands: and (c) conductivity change, i.e., the analyte binding "switches on" the conductivity of the surface-bound aptamer-DNA constructs. In each section, we will discuss the performance of these novel biosensors with representative examples reported in recent literature. (C) 2009 Elsevier B.V. All rights reserved.