Direct and Single-Molecule Visualization of the Solution-State Structures of G-Hairpin and G-Triplex Intermediates
Direct and Single-Molecule Visualization of the Solution-State Structures of G-Hairpin and G-Triplex Intermediates
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DOI：
10.1002/anie.201308903
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发表时间：
2014-04-14
影响因子：
16.6
通讯作者：
Sugiyama, Hiroshi
中科院分区：
文献类型：
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作者：
Rajendran, Arivazhagan;Endo, Masayuki;Sugiyama, Hiroshi
我们提出了 G-四链体折叠通路内中间体的直接单分子可视化，这是迄今为止采用的任何实验方法都无法实现的。使用 DNA 折纸作为结构控制和高速原子力显微镜 (HS-AFM) 直接可视化的新型工具，我们以纳米精度捕获了四分子反平行和 (3+1) 型 G-四链体中间体（例如 G-发夹和 G-三链体）前所未有的溶液态结构图像。以前任何直接或间接技术、溶液或固态、单分子或本体研究以及任何分辨率都没有报道过此类结构信息。根据我们的结果，我们提出了这些 G-四联体的折叠机制。
We present the direct and single-molecule visualization of the in-pathway intermediates of the G-quadruplex folding that have been inaccessible by any experimental method employed to date. Using DNA origami as a novel tool for the structural control and high-speed atomic force microscopy (HS-AFM) for direct visualization, we captured images of the unprecedented solution-state structures of a tetramolecular antiparallel and (3+1)-type G-quadruplex intermediates, such as G-hairpin and G-triplex, with nanometer precision. No such structural information was reported previously with any direct or indirect technique, solution or solid-state, single-molecule or bulk studies, and at any resolution. Based on our results, we proposed a folding mechanism of these G-quadruplexes.