A physical, chemical, and mechanical study of lumbar vertebrae from normal, ovariectomized, and nandrolone decanoate-treated cynomolgus monkeys (Macaca fascicularis)
A physical, chemical, and mechanical study of lumbar vertebrae from normal, ovariectomized, and nandrolone decanoate-treated cynomolgus monkeys (Macaca fascicularis)
复制标题
DOI：
10.1016/s8756-3282(00)00362-8
复制
发表时间：
2000-10-01
期刊：
影响因子：
4.1
通讯作者：
Rimnac, CM
中科院分区：
文献类型：
--
作者：
Gadeleta, SJ;Boskey, AL;Rimnac, CM
卵巢切除的食蟹猴先前已经作为绝经后骨质疏松症的非人灵长类动物模型进行了研究（杰罗姆等人，骨矿工9：527-540; 1994），在本研究中，傅里叶变换红外显微光谱（FTIRM）被用来验证在成熟的完整（INT）和卵巢切除（OVX）的猴椎骨骨矿物质的质量和数量的差异类似于在骨质疏松症和非患病的人骨中所观察到的。从三只ovx和三只INT成年猴（平均年龄8岁）的每个椎骨的15个骨小梁获得FTIRM光谱。这些光谱进行了比较，从3个“正常”和11个绝经后骨质疏松的人类受试者的骨小梁和以前报道的骨细胞骨。虽然矿物质：基质比（矿物质含量）、碳酸盐：磷酸盐比和结晶度的变化对于正常人受试者髂嵴的骨小梁是典型的，但对于绝经后骨质疏松的人受试者的骨小梁，这些参数的值相对稳定。一般而言，绝经后骨质疏松人类受试者的骨小梁相对于对照组显示矿物质含量降低（1.0 +/- 0.5 vs. 2.9 +/- 0.6）、结晶度增加和碳酸盐：磷酸盐增加。同样，与INT组（6.2 +/-0.2; p小于或等于0.05）相比，卵巢切除猴的小梁骨显示出较低的矿物质含量（5.8 +/-0.2），并且含有更大/更完美的磷灰石晶体（结晶度增加），碳酸盐：磷酸盐比率增加。绝对值的变化可归因于部位差异（髂骨与椎骨）。为了了解矿物质特性对机械特性的重要性，使用来自另一组猴的猴L-3和L-4椎体的芯进行压缩测试。在卵巢切除术后立即用合成代谢类固醇癸酸诺龙（ND）治疗猴子，并持续24个月（ND组），或卵巢切除术后12个月开始（dND组），与OVX治疗组相比，增加了极限应力，尽管骨结构和机械性能存在很大的动物间差异，这些数据支持这一假设，卵巢切除的成年猴是绝经后骨质疏松症的一个很好的模型，并可用于评估的治疗方式。(Bone 27：541-650; 2000）（C）2000，Elsevier Science Inc. All rights reserved.
Ovariectomized cynomolgus monkeys have previously been investigated as a nonhuman primate model of postmenopausal osteoporosis (Jerome et al., Bone Miner 9:527-540; 1994), In the present study, Fourier transform infrared microspectroscopy (FTIRM) was used to verify that differences ih bone mineral quality and quantity in the vertebrae of mature intact (INT) and ovariectomized (ovx) monkeys were analogous to those seen in osteoporotic and nondiseased human bones. FTIRM spectra were acquired from 15 trabeculae per vertebra from three ovx and three INT adult monkeys (mean age 8 years). These spectra were compared with those of both trabecular and previously reported osteonal bone obtained from 3 "normal" and 11 postmenopausal osteoporotic human subjects. While variations in the mineral:matrix ratio (mineral content), carbonate:phosphate ratio, and crystallinity are typical for trabecular bone from iliac crests of normal human subjects, the values of these parameters were relatively static for trabecular bone from postmenopausal osteoporotic human subjects. In general, trabecular bone from postmenopausal osteoporotic human subjects exhibited decreased mineral content (1.0 +/- 0.5 vs. 2.9 +/- 0.6), increased crystallinity, and increased carbonate: phosphate relative to controls. Similarly, trabecular bone from ovariectomized monkeys exhibited lower mineral content (5.8 +/- 0.2) compared with the INT group (6.2 +/- 0.2; p less than or equal to 0.05) and contained larger/more perfect apatite crystals (increased crystallinity) with increased carbonate:phosphate ratios. Variations in absolute values were attributable to site differences (ilium vs. vertebrae). To appreciate the importance of mineral properties on mechanical properties, compression testing was performed using cores of monkey L-3 and L-4 vertebral bodies from a separate group of monkeys. Treating monkeys with the anabolic steroid nandrolone decanoate (ND) immediately after ovariectomy and for the next 24 months (ND group), or beginning 12 months after ovariectomy (dND group), increased the ultimate stress compared with an ovx treatment group, despite large interanimal variations in bone architecture and mechanical properties, These data support the hypothesis that ovariectomized adult monkeys are an excellent model for postmenopausal osteoporosis, and can be used for the evaluation of therapeutic modalities. (Bone 27:541-650; 2000) (C) 2000 by Elsevier Science Inc. All rights reserved.