EXPRESSION OF THE RECE GENE DURING INDUCTION OF THE SOS RESPONSE IN BACILLUS-SUBTILIS RECOMBINATION-DEFICIENT STRAINS
EXPRESSION OF THE RECE GENE DURING INDUCTION OF THE SOS RESPONSE IN BACILLUS-SUBTILIS RECOMBINATION-DEFICIENT STRAINS
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DOI：
10.1111/j.1365-2958.1989.tb00277.x
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发表时间：
1989-09-01
影响因子：
3.6
通讯作者：
ALONSO, JC
中科院分区：
文献类型：
--
作者：
GASSEL, M;ALONSO, JC
已经构建了recE基因与报告基因的转录融合体。发现recE的表达在用丝裂霉素C或萘啶酸损伤DNA后被诱导。这种特异性转录诱导被recE突变阻断。影响recB、recF和recL基因产物的突变显著降低诱导。然而，recE的去阻遏似乎不依赖于外切核酸酶V酶（也称为AddAB酶）的ATP依赖性DNA酶活性。
A transcriptional fusion of the recE gene to a reporter gene has been constructed. Expression of recE was found to be induced upon damage to DNA with either mitomycin C or nalidixic acid. This specific transcriptional induction is blocked by a recE mutation. Mutations affecting the recB, recF and recL gene products markedly reduced induction. However, derepression of recE seems to be independent of the ATP-dependent DNase activity of the exonuclease V enzyme (also called AddAB enzyme).