Neuropeptide FF inhibition of morphine effects in the rat hippocampus.
Neuropeptide FF inhibition of morphine effects in the rat hippocampus.
复制标题
神经肽 FF 抑制大鼠海马吗啡作用。
DOI：
10.1016/s0006-8993(96)01333-9
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发表时间：
1997
期刊：
影响因子：
2.9
通讯作者：
Lupica,CR
中科院分区：
文献类型：
--
作者：
Miller,KK;Lupica,CR
由于抑制中间神经元释放γ-氨基丁酸（GABA），阿片类药物对海马 CA1 锥体神经元具有兴奋作用。从电生理学角度来看，这种锥体细胞兴奋表现为细胞外记录的群体峰值的增加，而突触 GABA 释放的减少则表现为细胞内记录的抑制性突触后电位 (IPSP) 幅度的降低。最近的研究表明，阿片类药物的一些行为效应，如镇痛，可以被抗阿片类肽如神经肽 FF (NPFF) 抑制。在本研究中，我们利用海马对阿片类药物的反应来检查吗啡和 NPFF 之间的体外潜在相互作用。单独使用吗啡 (20–200 μM) 会引起可逆的浓度依赖性群体峰值增加和 IPSP 减少。在细胞外实验中，单独使用 NPFF (1 μM) 对群体峰值没有影响，但显着且浓度依赖性地抑制吗啡诱导的这些反应的增加。细胞内实验表明，虽然NPFF对IPSP振幅或其他锥体神经元膜特性（膜电位、输入电阻、后超极化、动作电位频率）没有影响，但它显着减少吗啡引起的IPSP振幅降低。这些结果表明，NPFF 可以减弱吗啡对海马体中群体峰值和 IPSP 的影响，并表明这种效应发生在突触前部位，可能涉及 GABA 能中间神经元。
Opioids have an excitatory effect on CA1 pyramidal neurons in the hippocampus due to the inhibition of γ-aminobutyric acid (GABA) release from interneurons. Electrophysiologically, this pyramidal cell excitation is manifest as an increase in extracellularly recorded population spikes, while the reduction in synaptic GABA release is manifest as a decrease in the amplitude of intracellularly recorded inhibitory postsynaptic potentials (IPSPs). Recent studies suggest that some of the behavioral effects of opioids, such as antinociception, can be inhibited antiopioid peptides such as neuropeptide FF (NPFF). In the present study, we have used the hippocampal response to opioids to examine the potential interactions between morphine and NPFF in vitro. Morphine alone (20–200 μM) caused reversible concentration-dependent increases in population spikes and decreases in IPSPs. In extracellular experiments, NPFF (1 μM) alone had no effect on population spikes, but significantly and concentration-dependently inhibited the morphine-induced increases in these responses. Intracellular experiments indicated that while NPFF had no effect on IPSP amplitude, or other pyramidal neurons membrane properties (membrane potential, input resistance, afterhyperpolarization, action potential frequency), it significantly reduced the decrease in IPSP amplitude caused by morphine. These results demonstrate that NPFF can attenuate the effects of morphine on population spikes and IPSPs in the hippocampus, and suggest that this effect occurs at a presynaptic site, possibly involving GABAergic interneurons.