The genome and transcriptome of Japanese flounder provide insights into flatfish asymmetry
The genome and transcriptome of Japanese flounder provide insights into flatfish asymmetry
复制标题
日本比目鱼的基因组和转录组为比目鱼不对称性提供了见解
DOI：
10.1038/ng.3732
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发表时间：
2017-01-01
期刊：
影响因子：
30.8
通讯作者：
Chen, Songlin
中科院分区：
文献类型：
--
作者：
Shao, Changwei;Bao, Baolong;Chen, Songlin
比目鱼具有脊椎动物中最极端的不对称身体形态。在变态过程中，一只眼睛迁移到颅骨的对侧，这种迁移伴随着广泛的颅面转变和不平衡的身体色素沉着的同时发展。这种发育和生理创新的演变仍然是个谜。两种比目鱼的比较基因组学和变态过程中的转录组分析表明甲状腺激素和视黄酸信号传导以及光转导途径的作用。我们证明视黄酸对于建立不对称色素沉着以及通过与甲状腺激素的相互作用来调节眼睛迁移至关重要。来自皮肤光转导途径的视觉视蛋白的意外表达转化了照明差异并产生视黄酸梯度，这是不对称性产生的基础。确定这种独特发育过程的遗传基础可以回答有关不对称进化起源的长期存在的问题，同时也可以深入了解控制脊椎动物体形的机制。
Flatfish have the most extreme asymmetric body morphology of vertebrates. During metamorphosis, one eye migrates to the contralateral side of the skull, and this migration is accompanied by extensive craniofacial transformations and simultaneous development of lopsided body pigmentation,,,,. The evolution of this developmental and physiological innovation remains enigmatic. Comparative genomics of two flatfish and transcriptomic analyses during metamorphosis point to a role for thyroid hormone and retinoic acid signaling, as well as phototransduction pathways. We demonstrate that retinoic acid is critical in establishing asymmetric pigmentation and, via cross-talk with thyroid hormones, in modulating eye migration. The unexpected expression of the visual opsins from the phototransduction pathway in the skin translates illumination differences and generates retinoic acid gradients that underlie the generation of asymmetry. Identifying the genetic underpinning of this unique developmental process answers long-standing questions about the evolutionary origin of asymmetry, but it also provides insight into the mechanisms that control body shape in vertebrates.