Microfluidic device coupled with a microfabricated oxygen electrode for the measurement of bactericidal activity of neutrophil-like cells.
Microfluidic device coupled with a microfabricated oxygen electrode for the measurement of bactericidal activity of neutrophil-like cells.
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DOI：
10.1016/j.aca.2017.07.049
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发表时间：
2017-09
影响因子：
6.2
通讯作者：
A. Yamagishi;K. Tanabe;M. Yokokawa;Y. Morimoto;M. Kinoshita;H. Suzuki
中科院分区：
文献类型：
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作者：
A. Yamagishi;K. Tanabe;M. Yokokawa;Y. Morimoto;M. Kinoshita;H. Suzuki
采用微流控装置与微制克拉克型氧电极相结合的方法，测定了HL-60细胞分化为中性粒细胞样细胞的杀菌活性。将中性粒样细胞和大肠杆菌细胞培养在同一种介质中，并将其引入装置的流道中。用溶氧量的变化来衡量大肠杆菌呼吸活性的变化。随着中性粒细胞样细胞活性的增加，大肠杆菌的清除率增加。伴随而来的大肠杆菌数量的减少减少了溶解氧的消耗。这些变化实际上是作为产生电流的变化而观察到的。在与干扰素-γ激活的和未激活的中性粒细胞共同孵育的E.结肠细胞中，观察到溶氧浓度的变化明显不同。所需样本量不到10μ的L，1-2小时内即可得出结果，该装置或可用于评估影响中性粒细胞活性的心理应激。
A microfluidic device coupled with a microfabricated Clark-type oxygen electrode was used to measure the bactericidal activity of neutrophil-like cells differentiated from HL-60 cells. The neutrophil-like cells andEscherichia coli(E. coli) cells were cultured in the same medium, which was introduced into the flow channel of the device. Changes in the respiratory activity ofE. coliwere measured as changes in the consumption of dissolved oxygen. As the activity of the neutrophil-like cells increased, the rate of elimination ofE. coliincreased. The accompanying decrease in the number ofE. colireduced the consumption of dissolved oxygen. The changes were actually observed as changes in generated current. A distinct difference in changes in dissolved oxygen concentrations was observed betweenE. colicells co-incubated with IFN-γ-activated or non-activated neutrophil-like cells. The required sample volume was less than 10 μL, and results could be obtained within 1–2 h. The device may be useful for the assessment of psychological stresses that affect the activity of neutrophils.