Immunocytochemical localization and ontogenic development of a-melanocyte-stimulating hormone (α-MSH) in the brain of a pleuronectiform fish, barfin flounder
Immunocytochemical localization and ontogenic development of a-melanocyte-stimulating hormone (α-MSH) in the brain of a pleuronectiform fish, barfin flounder
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DOI：
10.1007/s00441-004-1058-4
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发表时间：
2005-04-01
影响因子：
3.6
通讯作者：
Yamamori, K
中科院分区：
文献类型：
--
作者：
Amano, M;Takahashi, A;Yamamori, K
α-黑素细胞刺激激素 (α-MSH) 是一种由阿黑皮素原翻译后加工衍生的垂体激素，参与硬骨鱼的背景适应。据报道，它还能抑制哺乳动物的食物摄入。在这里，我们检查了 α-MSH 在胸鳍鱼（Verasper moseri）的大脑和垂体中的免疫细胞化学定位，作为阐明 α-MSH 在大脑中可能功能的第一步。还研究了 α-MSH 系统的个体发育。在垂体中，α-MSH 免疫反应性 (ir) 细胞优先在中间部检测到。在大脑中，α-MSH-ir神经元胞体位于下丘脑基底外侧结节核，α-MSH-ir纤维主要位于端脑、下丘脑和中脑。 α-MSH-ir 神经元体体没有将轴突投射到垂体。 α-MSH-ir 神经元与那些对黑色素浓缩激素产生免疫反应的神经元不同。分别在孵化后 1 天和 5 天首次检测到垂体中的 α-MSH 细胞和大脑中的 α-MSH-ir 神经元体细胞。孵化后 30 天，α-MSH-ir 细胞、神经元体和纤维的分布与成鱼相似。这些结果表明，α-MSH在大脑和垂体中的功能是不同的，α-MSH在鲽鱼的早期发育中发挥着生理作用。
alpha-Melanocyte-stimulating hormone (alpha-MSH) is a pituitary hormone derived by post-translational processing from proopiomelanocortin and is involved in background adaptation in teleost fish. It has also been reported to suppress food intake in mammals. Here, we examined the immunocytochemical localization of alpha-MSH in the brain and pituitary of a pleuronectiform fish, the barfin flounder (Verasper moseri), as a first step in unraveling the possible function of alpha-MSH in the brain. The ontogenic development of the alpha-MSH system was also studied. In the pituitary, alpha-MSH-immunoreactive (ir) cells were preferentially detected in the pars intermedia. In the brain, alpha-MSH-ir neuronal somata were located in the nucleus tuberis lateralis of the basal hypothalamus, and alpha-MSH-ir fibers were located mainly in the telencephalon, hypothalamus, and midbrain. alpha-MSH-ir neuronal somata did not project their axons to the pituitary. The alpha-MSH-ir neurons differed from those immunoreactive to melanin-concentrating hormone. alpha-MSH cells in the pituitary and alpha-MSH-ir neuronal somata in the brain were first detected 1 day and 5 days after hatching, respectively. The distribution of alpha-MSH-ir cells, neuronal somata, and fibers showed a pattern similar to that in adult fish 30 days after hatching. These results indicate that the functions of alpha-MSH in the brain and pituitary are different and that alpha-MSH plays physiological roles in the early development of the barfin flounder.