Inflammatory immune responses by Water-insoluble α-glucans
Inflammatory immune responses by Water-insoluble α-glucans
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DOI：
10.1177/154405910708600309
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发表时间：
2007-03-01
影响因子：
7.6
通讯作者：
Kawabata, S.
中科院分区：
文献类型：
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作者：
Okamoto, S.;Terao, Y.;Kawabata, S.
不溶于水的α-葡聚糖是由蔗糖通过变形链球菌的葡萄糖基转移酶-I合成的，在牙菌斑的形成中发挥重要作用。已知真菌细胞壁成分中的几种 β-葡聚糖和变形链球菌中的水溶性 α-葡聚糖可调节先天免疫。在本研究中，我们研究了水不溶性α-葡聚糖是否也诱导炎症先天免疫反应。我们的结果表明，由远缘链球菌合成的水不溶性α-葡聚糖激活小鼠腹腔渗出物巨噬细胞，产生促炎细胞因子。免疫反应不是由于蔗糖、水溶性α-葡聚糖、脂多糖或肽聚糖的污染造成的。此外，水不溶性α-葡聚糖刺激人单核细胞产生TNF-α和IL-8，而人多形核细胞被水不溶性α-葡聚糖激活，导致趋化性和过氧化氢产生。结果表明，水溶性α-葡聚糖可调节巨噬细胞和粒细胞诱导的炎症免疫反应，并表明这些α-葡聚糖诱导的炎症与牙周病的发生有关。
Water-insoluble alpha-glucans are synthesized from sucrose by glucosyltransferase-I of mutans streptococci and play an important role in the development of dental plaque. Several types of beta-glucans in fungal cell wall components and water-soluble alpha-glucans from Streptococcus mutans are known to modulate innate immunity. In the present study, we investigated whether water-insoluble alpha-glucans also induced inflammatory innate immune responses. Our results showed that water-insoluble alpha-glucans synthesized by Streptococcus sobrinus activated mouse peritoneal exudate macrophages to produce proinflammatory cytokines. The immunological responses were not due to contamination by sucrose, water-soluble alpha-glucan, lipopolysaccharide, or peptidoglycan. Furthermore, human monocytes stimulated by water-insoluble alpha-glucans produced TNF-alpha and IL-8, while human polymorphonuclear cells were activated by water-insoluble alpha-glucans, resulting in chemotaxis and hydrogen peroxide production. The results demonstrated that water-soluble alpha-glucans modulate macrophage- and granulocyte-induced inflammatory immune responses, and suggest that inflammation induced by those alpha-glucans is associated with the development of periodontal diseases.