EFFECTS OF RECOMBINANT BOVINE SOMATOTROPIN, INSULIN-LIKE GROWTH FACTOR-I AND INSULIN ON THE PROLIFERATION OF BOVINE GRANULOSA-CELLS IN-VITRO
EFFECTS OF RECOMBINANT BOVINE SOMATOTROPIN, INSULIN-LIKE GROWTH FACTOR-I AND INSULIN ON THE PROLIFERATION OF BOVINE GRANULOSA-CELLS IN-VITRO
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DOI：
10.1677/joe.0.1390067
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发表时间：
1993-10-01
影响因子：
4
通讯作者：
WEBB, R
中科院分区：
文献类型：
--
作者：
GONG, JG;MCBRIDE, D;WEBB, R
Treatment of heifers with recombinant bovine somatotrophin (BST) significantly increases the population of small ovarian follicles and peripheral concentrations of somatotrophin, insulin-like growth factor-I (IGF-I) and insulin. To investigate the possible mechanism(s) involved in the action of BST on ovarian follicles, the effects of BST, IGF-I and insulin, given alone or in combination with either FSH or LH, on the proliferation of bovine granulosa cells in vitro were examined using a serum-free culture system. Bovine granulosa cells were obtained from antral follicles classified into three size categories according to diameter: small 10 mm. The proliferation of granulosa cells was assessed by the incorporation of [H-3]thymidine into the cultured cells.
