Dual Native Chemical Ligation at Lysine
Dual Native Chemical Ligation at Lysine
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DOI：
10.1021/ja905491p
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发表时间：
2009-09-30
影响因子：
15
通讯作者：
Liu, Chuan-Fa
中科院分区：
文献类型：
--
作者：
Yang, Renliang;Pasunooti, Kalyan Kumar;Liu, Chuan-Fa
在赖氨酸的γ-碳上引入的巯基在两个连续步骤中介导α-和ε-胺的稳健天然化学连接。然后脱硫得到最终产物，其中连接位点处的赖氨酸残基在其侧链上具有异肽键。该方法可用于合成在赖氨酸上含有特殊翻译后修饰的蛋白质。
A thiol group introduced on the gamma-carbon of lysine mediates robust native chemical Ligation at both the alpha- and epsilon-amines in two consecutive steps. Desulfurization then affords the final product, in which the lysine residue at the ligation site has an isopeptide bond on its side chain. The method is useful for the synthesis of proteins containing special post-translational modifications on lysine.